PMID- 12904464
OWN - NLM
STAT- MEDLINE
DCOM- 20030909
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 23
IP  - 18
DP  - 2003 Aug 6
TI  - Synergistic effects of brain-derived neurotrophic factor and chondroitinase ABC 
      on retinal fiber sprouting after denervation of the superior colliculus in adult 
      rats.
PG  - 7034-44
AB  - Damage to the adult CNS often causes devastating and permanent deficits because 
      of the limited capacity of the brain for anatomical reorganization. The finding 
      that collateral sprouting of uninjured fiber tracts mediates recovery of function 
      prompts the search for experimental strategies that stimulate axonal plasticity 
      after CNS trauma. Here we characterize treatments that promote the sprouting of 
      undamaged retinal afferents into the denervated superior colliculus (SC) after a 
      partial retinal lesion in the adult rat. Delivery of brain-derived neurotrophic 
      factor (BDNF) was performed to enhance the intrinsic potential of retinal 
      ganglion cells to reelongate their axons. Reduction of the neurite 
      growth-inhibitory properties of the adult SC was accomplished via treatment with 
      chondroitinase ABC (C-ABC), which degrades chondroitin sulfate proteoglycans. 
      Retinal axons were labeled via intraocular injections of fluorescently tagged 
      cholera toxin B subunit, and fiber sprouting within the denervated SC was 
      measured by quantitative laser-scanning confocal microscopy 1 week after the 
      retinal lesion. We found that both the administration of BDNF and the injection 
      of C-ABC induce significant sprouting of retinal afferents into the collicular 
      scotoma. Remarkably, the combined treatment with BDNF and C-ABC showed 
      synergistic effects on axon growth. Colocalization analysis with anti-synapsin 
      antibodies demonstrated synapse formation by the sprouting axons. These results 
      suggest that the combined treatment with BDNF and C-ABC can be relevant in 
      therapies for the repair of the damaged adult CNS.
FAU - Tropea, Daniela
AU  - Tropea D
AD  - Istituto di Neuroscienze del Consiglio Nazionale delle Ricerche, and Scuola 
      Normale Superiore, 56100 Pisa, Italy.
FAU - Caleo, Matteo
AU  - Caleo M
FAU - Maffei, Lamberto
AU  - Maffei L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - 9012-63-9 (Cholera Toxin)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Animals
MH  - Axons/drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Count
MH  - Cholera Toxin
MH  - Chondroitin ABC Lyase/*pharmacology
MH  - Chondroitin Sulfate Proteoglycans/metabolism
MH  - Denervation
MH  - Drug Administration Routes
MH  - Drug Synergism
MH  - Microglia/metabolism/pathology
MH  - Nerve Fibers/drug effects
MH  - Rats
MH  - Rats, Long-Evans
MH  - Retina/cytology/*drug effects/*physiology
MH  - Retinal Ganglion Cells/drug effects/physiology
MH  - Scotoma/physiopathology
MH  - Superior Colliculi/drug effects/*physiology
MH  - Synapses/drug effects/physiology
PMC - PMC6740661
EDAT- 2003/08/09 05:00
MHDA- 2003/09/10 05:00
PMCR- 2004/02/06
CRDT- 2003/08/09 05:00
PHST- 2003/08/09 05:00 [pubmed]
PHST- 2003/09/10 05:00 [medline]
PHST- 2003/08/09 05:00 [entrez]
PHST- 2004/02/06 00:00 [pmc-release]
AID - 23/18/7034 [pii]
AID - 10.1523/JNEUROSCI.23-18-07034.2003 [doi]
PST - ppublish
SO  - J Neurosci. 2003 Aug 6;23(18):7034-44. doi: 10.1523/JNEUROSCI.23-18-07034.2003.