PMID- 12905658
OWN - NLM
STAT- MEDLINE
DCOM- 20040624
LR  - 20181130
IS  - 1000-503X (Print)
IS  - 1000-503X (Linking)
VI  - 24
IP  - 4
DP  - 2002 Aug
TI  - [Expression and purification of gene transfer vehicle mediated by epidermal 
      growth factor receptor].
PG  - 381-4
AB  - OBJECTIVE: To create a gene transfer vehicle for targeting gene therapy of cancer 
      with epidermal growth factor receptor overexpressing. METHODS: Encoding sequences 
      of the first domain of histone gene (H1) and EGF C-loop (EGFc) were obtained by 
      PCR amplification. These two DNA fragments were ligated by EcoR I site, and 
      cloned and sequenced. E. coli expression vector of the fusion gene was then 
      constructed. The fusion protein H1EGFc was purified by specific band isolation 
      from SDS-PAGE. RESULTS: The molecular weight of purified protein was consistent 
      with the designed request. Its purity reached 94.02%. CONCLUSION: A fusion 
      protein H1EGFc was expressed and purified.
FAU - Fang, Hua-sheng
AU  - Fang HS
AD  - National Laboratory of Medical Molecular Biology, Institute of Basic Medical 
      Sciences, CAMS, PUMC, Beijing 100005, China.
FAU - Zhang, Lin
AU  - Zhang L
FAU - Lu, Sheng-dong
AU  - Lu SD
FAU - Zhang, Shu-zheng
AU  - Zhang SZ
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao
JT  - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
JID - 8006230
RN  - 0 (Histones)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - ErbB Receptors/biosynthesis/*genetics
MH  - Escherichia coli/genetics
MH  - Gene Transfer Techniques
MH  - Genetic Therapy
MH  - Genetic Vectors
MH  - Histones/biosynthesis/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Protein Engineering
MH  - Recombinant Fusion Proteins/biosynthesis/genetics/*isolation & purification
EDAT- 2003/08/09 05:00
MHDA- 2004/06/25 05:00
CRDT- 2003/08/09 05:00
PHST- 2003/08/09 05:00 [pubmed]
PHST- 2004/06/25 05:00 [medline]
PHST- 2003/08/09 05:00 [entrez]
PST - ppublish
SO  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Aug;24(4):381-4.