PMID- 12906716
OWN - NLM
STAT- MEDLINE
DCOM- 20031014
LR  - 20220311
IS  - 1096-2964 (Print)
IS  - 1096-2964 (Linking)
VI  - 4
IP  - 2
DP  - 2003 Summer
TI  - Influence of the TNF-alpha and TNF-beta polymorphisms upon infectious risk and 
      outcome in surgical intensive care patients.
PG  - 163-9
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is a well-documented central 
      inflammatory mediator in sepsis. Specific polymorphisms of the TNF-alpha and 
      TNF-beta genes (TNF2 and LTA + 250, respectively) have been suggested to 
      correlate with higher mortality in septic shock. This study sought to determine 
      whether these polymorphisms of the TNF-alpha and -beta genes are associated with 
      an increased risk of infection in an at-risk surgical intensive care population. 
      MATERIALS AND METHODS: Forty-four consecutive patients with systemic inflammatory 
      response syndrome were enrolled prospectively in the study. Genomic DNA was 
      isolated from whole blood samples using standard phenol/chloroform extraction 
      techniques. Specific fragments including the polymorphic sites of each gene were 
      amplified by polymerase chain reaction, and restriction enzyme digestions were 
      performed. Genotypes were determined by gel electrophoresis and confirmed by 
      direct sequencing. RESULTS: Eighty-six percent of the patients were TNF1 
      homozygotes (G:G at -308 of the TNF-alpha promoter region), whereas 9% of the 
      patients were homozygous for TNF2 (A:A). There was no difference in the incidence 
      of sepsis, septic shock, or mortality between patients bearing the various 
      alleles. Only 13.6% of the patients exhibited the G:G alleles for TNF-beta, 
      whereas the homozygous A:A was present in 45.4% of the patients. CONCLUSION: The 
      presence of the A allele at these polymorphic sites did not predispose critically 
      ill surgical patients to either infection or septic shock.
FAU - Calvano, Jacqueline E
AU  - Calvano JE
AD  - Department of Surgery, UMDNJ-Robert Wood Johnson Medical School, New Brunswick 
      08903, USA.
FAU - Um, John Y
AU  - Um JY
FAU - Agnese, Doreen M
AU  - Agnese DM
FAU - Hahm, Sae J
AU  - Hahm SJ
FAU - Kumar, Ashwini
AU  - Kumar A
FAU - Coyle, Susette M
AU  - Coyle SM
FAU - Calvano, Steve E
AU  - Calvano SE
FAU - Lowry, Stephen F
AU  - Lowry SF
LA  - eng
GR  - GM34695/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Surg Infect (Larchmt)
JT  - Surgical infections
JID - 9815642
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aged
MH  - Base Sequence
MH  - Critical Care
MH  - Critical Illness
MH  - Cross Infection/complications/genetics/immunology
MH  - Female
MH  - Genetic Predisposition to Disease/genetics
MH  - Humans
MH  - Lymphotoxin-alpha/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic/*genetics
MH  - Risk
MH  - Sepsis/etiology/*genetics/immunology
MH  - Shock, Septic/genetics/immunology
MH  - Systemic Inflammatory Response Syndrome/genetics/immunology
MH  - Treatment Outcome
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2003/08/09 05:00
MHDA- 2003/10/15 05:00
CRDT- 2003/08/09 05:00
PHST- 2003/08/09 05:00 [pubmed]
PHST- 2003/10/15 05:00 [medline]
PHST- 2003/08/09 05:00 [entrez]
AID - 10.1089/109629603766956951 [doi]
PST - ppublish
SO  - Surg Infect (Larchmt). 2003 Summer;4(2):163-9. doi: 10.1089/109629603766956951.