PMID- 12906744
OWN - NLM
STAT- MEDLINE
DCOM- 20030916
LR  - 20071114
IS  - 0897-7151 (Print)
IS  - 0897-7151 (Linking)
VI  - 20
IP  - 6
DP  - 2003 Jun
TI  - GDNF and BDNF alter the expression of neuronal NOS, c-Jun, and p75 and prevent 
      motoneuron death following spinal root avulsion in adult rats.
PG  - 603-12
AB  - In the present study, we examined the effects of glial cell line-derived 
      neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), ciliary 
      neurotrophic factor (CNTF), and insulin growth factor (IGF-1) on adult motoneuron 
      survival following spinal root avulsion. The expression of neuronal nitric oxide 
      synthase (nNOS), c-Jun, and the low-affinity neurotrophin receptor (P75) 
      following treatment with these neurotrophic factors was also examined. In control 
      animals, approximately 80% of spinal motoneurons were nNOS positive at 3 weeks 
      following the lesion, whereas in GDNF or BDNF treated animals no nNOS positive 
      motoneurons were found at the same time point. Following injury and treatment 
      with GDNF and BDNF increased numbers of motoneurons were c-Jun and P75 positive. 
      By 6 weeks following the lesion, only approximately 28% of motoneurons persisted 
      in control animals whereas about 90% of motoneurons survived injury following 
      treatment with either GDNF or BDNF. In contrast, CNTF and IGF-1 were ineffective 
      in either inhibiting nNOS expression or preventing motoneuron death. Our results 
      provide in vivo evidence that the survival of injured adult mammalian motoneurons 
      can be promoted by specific neurotrophic factors, and that this effect is 
      associated with inhibition of nNOS expression and up-regulation of c-Jun and P75 
      expression.
FAU - Wu, Wutian
AU  - Wu W
AD  - Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong, 
      China. wtwu@hkucc.hku.hk
FAU - Li, Linxi
AU  - Li L
FAU - Yick, Leung-Wah
AU  - Yick LW
FAU - Chai, Hong
AU  - Chai H
FAU - Xie, Yuanyun
AU  - Xie Y
FAU - Yang, Yi
AU  - Yang Y
FAU - Prevette, David M
AU  - Prevette DM
FAU - Oppenheim, Ronald W
AU  - Oppenheim RW
LA  - eng
GR  - NS 20402/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nos1 protein, rat)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology/therapeutic use
MH  - Cell Death/drug effects/physiology
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Male
MH  - Motor Neurons/drug effects/metabolism/pathology
MH  - Nerve Growth Factors/*pharmacology/therapeutic use
MH  - Nitric Oxide Synthase/*biosynthesis/genetics
MH  - Nitric Oxide Synthase Type I
MH  - Proto-Oncogene Proteins c-jun/*biosynthesis/genetics
MH  - Radiculopathy/drug therapy/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Nerve Growth Factor
MH  - Receptors, Nerve Growth Factor/*biosynthesis/genetics
EDAT- 2003/08/09 05:00
MHDA- 2003/09/17 05:00
CRDT- 2003/08/09 05:00
PHST- 2003/08/09 05:00 [pubmed]
PHST- 2003/09/17 05:00 [medline]
PHST- 2003/08/09 05:00 [entrez]
AID - 10.1089/089771503767168528 [doi]
PST - ppublish
SO  - J Neurotrauma. 2003 Jun;20(6):603-12. doi: 10.1089/089771503767168528.