PMID- 12907415
OWN - NLM
STAT- MEDLINE
DCOM- 20040107
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 285
IP  - 6
DP  - 2003 Dec
TI  - Cortisol alters carbonic anhydrase-mediated renal sulfate secretion.
PG  - R1430-8
AB  - Active transepithelial sulfate secretion rate by winter flounder renal proximal 
      tubule epithelium in primary culture (fPTC) is dependent on intracellular 
      carbonic anhydrase (CA) and enhanced by cortisol. To further evaluate this 
      relationship, a partial cDNA clone (327 bp) of carbonic anhydrase II (CAII) with 
      high sequence similarity to CAII from numerous species including fish, chicken, 
      and human was obtained from fPTCs. The majority of CA activity and CAII protein 
      was present in the cytosol of fPTCs; however, significant amounts of both (in 
      addition to SDS-resistant CA activity, i.e., CAIV-like isoform) were present in 
      concentrated plasma membranes. CAII from concentrated membranes migrated 
      differently than purified CAII on nondenaturing PAGE gels, suggesting that CAII 
      associates with another membrane component. Treatment of fPTCs with the 
      cell-soluble CA inhibitor methazolamide (100 microM) caused a 58% reduction in 
      active transepithelial SO4(2-) secretion. fPTCs that were previously cultured 
      under high-cortisol concentrations, when subjected to 5 days of low physiological 
      levels of cortisol, had decreased CA activity (28%), CAII protein abundance 
      (65%), and net active SO4(2-) secretion (28%), with no effect on epithelial 
      differentiation. Methazolamide and low-cortisol treatment in combination 
      inhibited net active SO4(2-) secretion 56%, which was not different than the 
      effect of methazolamide treatment alone. These data indicate that cortisol 
      directly increases renal CA activity, CAII protein abundance, and CA-dependent 
      SO4(2-) secretion in the marine teleost renal proximal tubule.
FAU - Pelis, Ryan M
AU  - Pelis RM
AD  - Department of Physiology and Neurobiology, U-4156, Univiversity of Connecticut, 
      3107 Horsebarn Hill Rd., Storrs, Connecticut 06269-4156, USA.
FAU - Goldmeyer, James E
AU  - Goldmeyer JE
FAU - Crivello, Joseph
AU  - Crivello J
FAU - Renfro, J Larry
AU  - Renfro JL
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20030807
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (RNA, Messenger)
RN  - 0 (Sulfates)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Acidosis/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport/drug effects
MH  - Carbonic Anhydrase II/genetics/*metabolism
MH  - Flounder
MH  - Hydrocortisone/*pharmacology
MH  - Kidney Tubules, Proximal/drug effects/*enzymology/*metabolism
MH  - Molecular Sequence Data
MH  - Organ Culture Techniques
MH  - RNA, Messenger/analysis
MH  - Sulfates/*metabolism
EDAT- 2003/08/09 05:00
MHDA- 2004/01/08 05:00
CRDT- 2003/08/09 05:00
PHST- 2003/08/09 05:00 [pubmed]
PHST- 2004/01/08 05:00 [medline]
PHST- 2003/08/09 05:00 [entrez]
AID - 00331.2003 [pii]
AID - 10.1152/ajpregu.00331.2003 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2003 Dec;285(6):R1430-8. doi: 
      10.1152/ajpregu.00331.2003. Epub 2003 Aug 7.