PMID- 12910266
OWN - NLM
STAT- MEDLINE
DCOM- 20030930
LR  - 20171116
IS  - 1529-2908 (Print)
IS  - 1529-2908 (Linking)
VI  - 4
IP  - 9
DP  - 2003 Sep
TI  - Genetic tagging shows increased frequency and longevity of antigen-presenting, 
      skin-derived dendritic cells in vivo.
PG  - 907-12
AB  - Dendritic cells (DCs) are key regulators of immune responses that activate naive 
      antigen-specific T lymphocytes. In draining lymph nodes, antigen-bearing DCs are 
      reported to be rare and short-lived. How such small numbers of short-lived DCs 
      can activate rare antigen-specific T cells is unclear. Here we show that after 
      immunization of mouse skins by gene gun, the number of antigen-bearing DCs that 
      migrate to draining lymph node is 100-fold higher than previously estimated and 
      that they persist for approximately 2 weeks. The substantial frequency and 
      longevity of DCs in situ ensures ample antigen presentation and stimulation for 
      the rare antigen-specific T cells in draining lymph nodes.
FAU - Garg, Sanjay
AU  - Garg S
AD  - Department of Microbiology and Immunology, Vaccine Research Center, Yerkes 
      National Primate Research Center, Emory University, 954 Gatewood Road, Atlanta, 
      Georgia 30322, USA.
FAU - Oran, Alp
AU  - Oran A
FAU - Wajchman, Janine
AU  - Wajchman J
FAU - Sasaki, Shin
AU  - Sasaki S
FAU - Maris, Charles H
AU  - Maris CH
FAU - Kapp, Judith A
AU  - Kapp JA
FAU - Jacob, Joshy
AU  - Jacob J
LA  - eng
GR  - AI R01524751/AI/NIAID NIH HHS/United States
GR  - EY13459/EY/NEI NIH HHS/United States
GR  - F32 EY06985/EY/NEI NIH HHS/United States
GR  - P30 EYO06360/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030810
PL  - United States
TA  - Nat Immunol
JT  - Nature immunology
JID - 100941354
RN  - 0 (Antigens, Viral)
RN  - 0 (CD11c Antigen)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (immediate-early proteins, cytomegalovirus)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/genetics
MH  - Biolistics
MH  - CD11c Antigen/immunology
MH  - Cell Movement/immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Immediate-Early Proteins/genetics
MH  - Immunization/methods
MH  - Kinetics
MH  - Lymph Nodes/cytology/immunology
MH  - Mice
MH  - Mice, Transgenic
MH  - Phenotype
MH  - Promoter Regions, Genetic
MH  - Skin/cytology/*immunology
MH  - T-Lymphocytes/immunology
EDAT- 2003/08/12 05:00
MHDA- 2003/10/01 05:00
CRDT- 2003/08/12 05:00
PHST- 2003/04/18 00:00 [received]
PHST- 2003/07/10 00:00 [accepted]
PHST- 2003/08/12 05:00 [pubmed]
PHST- 2003/10/01 05:00 [medline]
PHST- 2003/08/12 05:00 [entrez]
AID - ni962 [pii]
AID - 10.1038/ni962 [doi]
PST - ppublish
SO  - Nat Immunol. 2003 Sep;4(9):907-12. doi: 10.1038/ni962. Epub 2003 Aug 10.