PMID- 12911419
OWN - NLM
STAT- MEDLINE
DCOM- 20031224
LR  - 20190823
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 58
IP  - 9
DP  - 2003 Sep
TI  - Effect of anti-nerve growth factor on early and late airway responses in allergic 
      rats.
PG  - 900-4
AB  - BACKGROUND: The increased production of nerve growth factor (NGF) has been 
      associated with allergen-induced airway hyperresponsiveness and enhanced airway 
      inflammation in experimental models of asthma. The aim of this study was to 
      investigate whether a local application of anti-NGF to the lungs may affect the 
      allergen-specific early (EAR) and late (LAR) airway responses to ovalbumin (Ova) 
      of Ova-sensitized brown Norway rats. METHODS: Rats were sensitized systemically 
      with Ova and were boosted twice intratracheally with Ova aerosol using a 
      microsprayer. Two hours before every boost, the animals were pretreated either 
      with aerosolized anti-NGF or with a control antibody. On day 21, all animals were 
      challenged with inhalational Ova aerosol and pulmonary resistance was recorded in 
      anesthetized, orotracheally intubated animals during the early and late asthmatic 
      responses. In addition, differential cell counts from bronchoalveolar lavage and 
      serum immunoglobulin E (IgE) levels were determined 48 h post-Ova challenge. 
      RESULTS: Pretreatment with anti-NGF significantly attenuated the EAR but had no 
      significant effect on the LAR. Serum IgE levels and inflammatory cell influx into 
      the lungs were not affected by anti-NGF pretreatment. CONCLUSION: The data from 
      this study suggest that NGF is directly involved in the development of the EAR 
      without affecting the inflammatory airway response or LAR.
FAU - Glaab, T
AU  - Glaab T
AD  - Department of Immunology and Allergology, Fraunhofer Institute of Toxicology and 
      Experimental Medicine, Nikolai-Fuchs-Strasse 1, D-30625 Hannover, Germany.
FAU - Hoymann, H G
AU  - Hoymann HG
FAU - Hecht, M
AU  - Hecht M
FAU - Korolewitz, R
AU  - Korolewitz R
FAU - Tschernig, T
AU  - Tschernig T
FAU - Hohlfeld, J M
AU  - Hohlfeld JM
FAU - Krug, N
AU  - Krug N
FAU - Braun, A
AU  - Braun A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Aerosols)
RN  - 0 (Antibodies)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Aerosols
MH  - Animals
MH  - Antibodies/*administration & dosage
MH  - Hypersensitivity, Delayed/etiology/*physiopathology
MH  - Hypersensitivity, Immediate/etiology/*physiopathology
MH  - Lung/*immunology/pathology
MH  - Nerve Growth Factor/*immunology
MH  - Ovalbumin/administration & dosage
MH  - Pneumonia/etiology/pathology
MH  - Rats
MH  - Rats, Inbred BN
MH  - Respiratory Hypersensitivity/complications/etiology/*physiopathology
EDAT- 2003/08/13 05:00
MHDA- 2003/12/25 05:00
CRDT- 2003/08/13 05:00
PHST- 2003/08/13 05:00 [pubmed]
PHST- 2003/12/25 05:00 [medline]
PHST- 2003/08/13 05:00 [entrez]
AID - 208 [pii]
AID - 10.1034/j.1398-9995.2003.00208.x [doi]
PST - ppublish
SO  - Allergy. 2003 Sep;58(9):900-4. doi: 10.1034/j.1398-9995.2003.00208.x.