PMID- 12911533
OWN - NLM
STAT- MEDLINE
DCOM- 20040414
LR  - 20131121
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 64
IP  - 3
DP  - 2003 Sep
TI  - Oxidative stress and kidney dysfunction due to ischemia/reperfusion in rat: 
      attenuation by dehydroepiandrosterone.
PG  - 836-43
AB  - BACKGROUND: The pathogenesis of ischemia/reperfusion (I/R) involves generation of 
      reactive oxygen and nitrogen species. This in vivo study investigates the effect 
      of dehydroepiandrosterone (DHEA), a physiologic steroid with antioxidant 
      properties, on oxidative balance and renal dysfunctions induced by monolateral 
      I/R. METHODS: Normal and DHEA-treated rats (4 mg/day x 21 days, orally) were 
      subjected to monolateral renal I/R (30 minutes/6 hours). The oxidative state was 
      determined by measuring hydrogen peroxide level and activities of 
      glutathione-peroxidase, catalase, and superoxide dismutase. Tumor necrosis 
      factor-alpha (TNF-alpha) and nitric oxide production and inducible nitric oxide 
      synthase (iNOS) levels were also measured. Hydroxynonenal content was used to 
      probe lipid peroxidation. Functional parameters determined were creatinine levels 
      and Na/K-ATPase activity. Immunohistochemical and morphologic studies were also 
      performed. RESULTS: A markedly pro-oxidant state was evident in the kidney of 
      rats subjected to I/R. Both hydrogen peroxide and reactive nitrogen species 
      (nitric oxide and iNOS) increased, whereas antioxidants decreased. Oxidant 
      species induce TNF-alpha increase, which, in turn, produces lipoperoxidative 
      processes, as documented by the increased hydroxynonenal (HNE) level. As final 
      result, impaired renal functionality, hydropic degeneration, and vacuolization of 
      proximal convolute tubules were observed in kidneys of I/R rats. DHEA 
      pretreatment improved the parameters considered. CONCLUSION: I/R induces 
      oxidative stress and consequently damages the proximal convolute renal tubules. 
      Rats supplemented with DHEA and subjected to I/R had reduced pro-oxidant state, 
      oxidative damage, and improved renal functionality, indicating an attenuation of 
      oxidative injury and dysfunctions mediated by I/R.
FAU - Aragno, Manuela
AU  - Aragno M
AD  - Department of Experimental Medicine and Oncology, General Pathology Section, 
      University of Turin, Turin, Italy.
FAU - Cutrin, Juan Carlos
AU  - Cutrin JC
FAU - Mastrocola, Raffaella
AU  - Mastrocola R
FAU - Perrelli, Maria-Giulia
AU  - Perrelli MG
FAU - Restivo, Francesca
AU  - Restivo F
FAU - Poli, Giuseppe
AU  - Poli G
FAU - Danni, Oliviero
AU  - Danni O
FAU - Boccuzzi, Giuseppe
AU  - Boccuzzi G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Antioxidants)
RN  - 459AG36T1B (Dehydroepiandrosterone)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Dehydroepiandrosterone/*pharmacology
MH  - Immunohistochemistry
MH  - Kidney/drug effects/pathology/*physiopathology
MH  - Male
MH  - *Oxidative Stress
MH  - Rats
MH  - Rats, Wistar
MH  - *Renal Circulation
MH  - Reperfusion Injury/pathology/*physiopathology
EDAT- 2003/08/13 05:00
MHDA- 2004/04/15 05:00
CRDT- 2003/08/13 05:00
PHST- 2003/08/13 05:00 [pubmed]
PHST- 2004/04/15 05:00 [medline]
PHST- 2003/08/13 05:00 [entrez]
AID - S0085-2538(15)49404-9 [pii]
AID - 10.1046/j.1523-1755.2003.00152.x [doi]
PST - ppublish
SO  - Kidney Int. 2003 Sep;64(3):836-43. doi: 10.1046/j.1523-1755.2003.00152.x.