PMID- 12911541
OWN - NLM
STAT- MEDLINE
DCOM- 20040414
LR  - 20071114
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 64
IP  - 3
DP  - 2003 Sep
TI  - Rat glomerular epithelial cells produce and bear factor H on their surface that 
      is up-regulated under complement attack.
PG  - 914-22
AB  - BACKGROUND: Factor H is a potent complement inhibitory molecule that is primarily 
      produced by the liver and appears in plasma as a soluble protein. Yet there is 
      evidence that other cells, including those in the kidney, can produce factor H, 
      and that it can be cell-associated as well as present as a plasma protein. Here 
      we studied factor H in rat glomerular epithelial cells (GEC). METHODS: A 
      polyclonal antibody to factor H was used to identify factor H protein. A 
      polymerase chain reaction (PCR)-based strategy was utilized to clone the 
      full-length cDNA of GEC factor H. The relative quantity of factor H mRNA was 
      measured by quantitative reverse transcription (RT)-PCR in cultured GEC exposed 
      to complement activation and in the passive Heymann nephritis (PHN) model of 
      membranous nephropathy. RESULTS: By immunofluorescence microscopy, factor H 
      protein was present on the plasma membranes of cultured GEC. Based upon Western 
      blot studies, this appeared to be the full-length 150 kD factor H protein. Factor 
      H cDNA cloned from GEC was identical to the newly deposited sequence for rat 
      liver factor H cDNA. In cultured GEC in which complement was activated, factor H 
      mRNA increased over time. Similarly, in the PHN model in which complement was 
      activated on GEC in vivo, factor H mRNA and protein also increased over time. 
      CONCLUSION: Cultured GEC and glomeruli express factor H mRNA and protein. As 
      modeled both in vitro and in vivo in the rat, factor H is up-regulated in 
      membranous nephropathy. This is likely to be a direct response of GEC to 
      complement attack and may represent a protective response of this cell.
FAU - Ren, Guohui
AU  - Ren G
AD  - Section of Nephrology, The University of Chicago, Chicago, Illinois, USA.
FAU - Doshi, Mona
AU  - Doshi M
FAU - Hack, Bradley K
AU  - Hack BK
FAU - Alexander, Jessy J
AU  - Alexander JJ
FAU - Quigg, Richard J
AU  - Quigg RJ
LA  - eng
GR  - R01DK48173/DK/NIDDK NIH HHS/United States
GR  - T32DK07510/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 80295-65-4 (Complement Factor H)
SB  - IM
MH  - Animals
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Complement Activation/*physiology
MH  - Complement Factor H/genetics/*metabolism
MH  - DNA, Complementary/genetics
MH  - Epithelial Cells/metabolism
MH  - Kidney Glomerulus/cytology/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Up-Regulation
EDAT- 2003/08/13 05:00
MHDA- 2004/04/15 05:00
CRDT- 2003/08/13 05:00
PHST- 2003/08/13 05:00 [pubmed]
PHST- 2004/04/15 05:00 [medline]
PHST- 2003/08/13 05:00 [entrez]
AID - S0085-2538(15)49412-8 [pii]
AID - 10.1046/j.1523-1755.2003.00188.x [doi]
PST - ppublish
SO  - Kidney Int. 2003 Sep;64(3):914-22. doi: 10.1046/j.1523-1755.2003.00188.x.