PMID- 12913147
OWN - NLM
STAT- MEDLINE
DCOM- 20031021
LR  - 20240410
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 132
IP  - 4
DP  - 2003 Aug
TI  - Overexpression of the disease resistance gene Pto in tomato induces gene 
      expression changes similar to immune responses in human and fruitfly.
PG  - 1901-12
AB  - The Pto gene encodes a serine/threonine protein kinase that confers resistance in 
      tomato (Lycopersicon esculentum) to Pseudomonas syringae pv tomato strains that 
      express the type III effector protein AvrPto. Constitutive overexpression of Pto 
      in tomato, in the absence of AvrPto, activates defense responses and confers 
      resistance to several diverse bacterial and fungal plant pathogens. We have used 
      a series of gene discovery and expression profiling methods to examine the effect 
      of Pto overexpression in tomato leaves. Analysis of the tomato expressed sequence 
      tag database and suppression subtractive hybridization identified 600 genes that 
      were potentially differentially expressed in Pto-overexpressing tomato plants 
      compared with a sibling line lacking Pto. By using cDNA microarrays, we verified 
      changes in expression of many of these genes at various time points after 
      inoculation with P. syringae pv tomato (avrPto) of the resistant 
      Pto-overexpressing line and the susceptible sibling line. The combination of 
      these three approaches led to the identification of 223 POR (Pto overexpression 
      responsive) genes. Strikingly, 40% of the genes induced in the Pto-overexpressing 
      plants previously have been shown to be differentially expressed during the human 
      (Homo sapiens) and/or fruitfly (Drosophila melanogaster) immune responses.
FAU - Mysore, Kirankumar S
AU  - Mysore KS
AD  - Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73402, 
      USA. kmysore@noble.org
FAU - D'Ascenzo, Mark D
AU  - D'Ascenzo MD
FAU - He, Xiaohua
AU  - He X
FAU - Martin, Gregory B
AU  - Martin GB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Plant Proteins)
RN  - EC 2.7.1.- (Pto protein, Lycopersicon)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Drosophila melanogaster/genetics/*immunology
MH  - Expressed Sequence Tags
MH  - *Gene Expression Profiling
MH  - *Gene Expression Regulation, Plant
MH  - Genes, Plant/genetics
MH  - Humans
MH  - Solanum lycopersicum/*genetics/immunology/*microbiology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Plant Leaves/genetics/microbiology
MH  - *Plant Proteins
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Serine-Threonine Kinases/*genetics/*metabolism
MH  - Signal Transduction
PMC - PMC181276
EDAT- 2003/08/13 05:00
MHDA- 2003/10/22 05:00
PMCR- 2004/08/01
CRDT- 2003/08/13 05:00
PHST- 2003/08/13 05:00 [pubmed]
PHST- 2003/10/22 05:00 [medline]
PHST- 2003/08/13 05:00 [entrez]
PHST- 2004/08/01 00:00 [pmc-release]
AID - 1321901 [pii]
AID - 10.1104/pp.103.022731 [doi]
PST - ppublish
SO  - Plant Physiol. 2003 Aug;132(4):1901-12. doi: 10.1104/pp.103.022731.