PMID- 12913251
OWN - NLM
STAT- MEDLINE
DCOM- 20040407
LR  - 20220408
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 26
IP  - 8
DP  - 2003 Aug
TI  - Virus concentration using sulfonated magnetic beads to improve sensitivity in 
      nucleic acid amplification tests.
PG  - 1065-9
AB  - To enhance the sensitivity of virus detection by polymerase chain reaction (PCR) 
      and reverse-transcriptional (RT)-PCR, we developed a novel virus-concentration 
      method using sulfonated (SO-) magnetic beads in the presence of divalent cations. 
      In the presence of either Zn(2+) or Cu(2+) ions, we showed that SO-magnetic beads 
      were able to concentrate non-enveloped model viruses, such as porcine parvovirus 
      (PPV) and poliovirus, which were not concentrated by polyethyleneimine 
      (PEI)-magnetic beads.(1)) Using the SO-magnetic beads, the sensitivity of virus 
      genome detection by PCR or RT-PCR can be enhanced. Therefore, an efficient virus 
      concentration method using either SO-magnetic beads or PEI-magnetic beads 
      enhances the sensitivity of virus detection by PCR or RT-PCR.
FAU - Iwata, Akiko
AU  - Iwata A
AD  - The National Institute of Health Sciences, Tokyo, Japan.
FAU - Satoh, Koei
AU  - Satoh K
FAU - Murata, Mitsuhiro
AU  - Murata M
FAU - Hikata, Mikio
AU  - Hikata M
FAU - Hayakawa, Takao
AU  - Hayakawa T
FAU - Yamaguchi, Teruhide
AU  - Yamaguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Sulfonic Acids)
SB  - IM
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Magnetics
MH  - *Microspheres
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Sulfonic Acids/*analysis
MH  - Swine
MH  - Vero Cells
MH  - Viruses/genetics/*isolation & purification
EDAT- 2003/08/13 05:00
MHDA- 2004/04/08 05:00
CRDT- 2003/08/13 05:00
PHST- 2003/08/13 05:00 [pubmed]
PHST- 2004/04/08 05:00 [medline]
PHST- 2003/08/13 05:00 [entrez]
AID - 10.1248/bpb.26.1065 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2003 Aug;26(8):1065-9. doi: 10.1248/bpb.26.1065.