PMID- 12913875
OWN - NLM
STAT- MEDLINE
DCOM- 20031219
LR  - 20220408
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 23
IP  - 8
DP  - 2003 Aug
TI  - Comparison between fluorescence in situ hybridization (FISH) and 
      quantitative-fluorescent polymerase chain reaction (QF-PCR) for the detection of 
      aneuploidies in single blastomeres.
PG  - 678-84
AB  - OBJECTIVES: The aim of our investigation was to compare the efficiencies of the 
      fluorescence in situ hybridization (FISH) and the quantitative-fluorescent PCR 
      (QF-PCR) methods for the detection of sexing and numerical chromosome disorders 
      in single blastomeres collected from the same preimplantation human embryos. 
      METHODS: FISH analysis was carried out on 145 blastomeres from the 79 surplus 
      embryos with probes specific for chromosomes 13, 18, 21, X, and Y. QF-PCR was 
      performed with each one or two of the primers specific for the same chromosomes 
      on 151 blastomeres from the same embryos obtained from patients undergoing IVF 
      treatment. RESULTS: Analyses were possible on 135 blastomeres (93%) by FISH and 
      on 117 blastomeres (77%) by QF-PCR. Of 65 embryos, which could be analyzed by 
      both methods, 20 embryos (31%) were diagnosed as abnormal. CONCLUSION: The 
      present study shows that FISH tests are more accurate than QF-PCR assays for the 
      detection of numerical chromosome disorders when performed on single blastomeres.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Sato, Takeshi
AU  - Sato T
AD  - Department of Obstetrics and Gynaecology, Graduate School of Medical Science, 
      Nagoya City University, Nagoya, Japan. og.sato@med.nagoya-cu.ac.jp
FAU - Ikuta, Katsuo
AU  - Ikuta K
FAU - Sherlock, Jon
AU  - Sherlock J
FAU - Adinolfi, Matteo
AU  - Adinolfi M
FAU - Suzumori, Kaoru
AU  - Suzumori K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Aneuploidy
MH  - Blastomeres/*ultrastructure
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Monosomy
MH  - Mosaicism
MH  - Polymerase Chain Reaction/*methods
MH  - Preimplantation Diagnosis/*methods
MH  - Trisomy
EDAT- 2003/08/13 05:00
MHDA- 2003/12/20 05:00
CRDT- 2003/08/13 05:00
PHST- 2003/08/13 05:00 [pubmed]
PHST- 2003/12/20 05:00 [medline]
PHST- 2003/08/13 05:00 [entrez]
AID - 10.1002/pd.660 [doi]
PST - ppublish
SO  - Prenat Diagn. 2003 Aug;23(8):678-84. doi: 10.1002/pd.660.