PMID- 12914287
OWN - NLM
STAT- MEDLINE
DCOM- 20040129
LR  - 20061115
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 32
IP  - 3
DP  - 2003 May
TI  - [Studies on fluorescence in situ hybridization with group-specific 16S rRNA-based 
      probes in analysis of human colonic microflora].
PG  - 232-5
AB  - In order to establish the optional experimental conditions of fluorescence in 
      situ hybridization (FISH) for analysis of colonic microflora, and analyze the 
      stability and validation of the technique, fresh fecal samples were collected 
      from the volunteers and five group-specific 16S rRNA-targeted oligonucleotide 
      probes were used, and the effects of keeping time and temperature of fresh fecal 
      samples after collected, the centrifugal speed and time, the storage time of 
      paraformaldehyde-stocks of samples on the results were analyzed. The stability 
      and the validation were assessed by CV values. Results showed that: (1) The 
      optional experimental conditions were that the fresh fecal samples were not kept 
      for more than 12 hours at 4 degrees C, the centrifugal speed of samples was 700 g 
      for 2 minutes, and the paraformaldehyde-stocks were not kept for more than 5 
      months at low temperature (-80 degrees C) before analysis; (2) The method had 
      good stability and could detect the differences of colonic microflora composition 
      between objects at a significant level.
FAU - Zhong, Yan
AU  - Zhong Y
AD  - Huaxi School of Public Health, Sichuan University, Chengdu 610041, China.
FAU - Huang, Chengyu
AU  - Huang C
FAU - Yin, Wenya
AU  - Yin W
FAU - Vonk, R J
AU  - Vonk RJ
FAU - Harmsen, H M J
AU  - Harmsen HM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adult
MH  - Colon/*microbiology
MH  - Colony Count, Microbial
MH  - Feces/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Oligonucleotide Probes
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Species Specificity
EDAT- 2003/08/14 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/08/14 05:00
PHST- 2003/08/14 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/08/14 05:00 [entrez]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2003 May;32(3):232-5.