PMID- 12915116
OWN - NLM
STAT- MEDLINE
DCOM- 20031017
LR  - 20211203
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 288
IP  - 2
DP  - 2003 Aug 15
TI  - Regulation of migration of primary prostate epithelial cells by secreted factors 
      from prostate stromal cells.
PG  - 246-56
AB  - Stromal-epithelial interactions, which regulate the migration of prostate 
      epithelial cells, play an important role in prostate development, prostatic 
      hyperplasia, and prostate cancer. The objective of this study was to determine 
      how the prostate stroma stimulates the migration of primary prostate epithelial 
      cells (PECs). In the Boyden chamber assay, PEC migration was strongly induced by 
      the conditioned medium of primary prostate stromal cells (PSC-CM). Stimulation of 
      PEC migration depended on the concerted action of adhesion and motility factors 
      in the PSC-CM. Immobilized proteins from PSC-CM mediated adhesion, spreading, and 
      head-to-tail polarization of PECs. Migration induced by immobilized PSC-CM 
      proteins was significantly increased by hepatocyte growth factor/scatter factor 
      (HGF/SF). Inhibition of P13-kinase or Src-family kinases, but not MEK or PLCchi, 
      abolished migration in the Boyden chamber assay. Consistent with their concerted 
      activity in migration assays, the combination of adhesion and motility factors 
      was required for efficient activation of the P13-kinase/Akt pathway. HGF/SF in 
      the PSC-CM was the principal stimulator of the P13-kinase/Akt pathway and an 
      important mediator of PSC-CM-induced PEC migration. In conclusion, our data show 
      that the migration of primary PECs is regulated by the P13-kinase and Src-family 
      kinase signaling pathways and that the activation of the P13-kinase pathway 
      requires adhesion and motility factors from the prostate stroma.
FAU - You, Xueke
AU  - You X
AD  - Department of Pathology, Weill Medical College of Cornell University, New York, 
      NY 10021, USA.
FAU - Yu, Hsiao-Man
AU  - Yu HM
FAU - Cohen-Gould, Leona
AU  - Cohen-Gould L
FAU - Cao, Brian
AU  - Cao B
FAU - Symons, Marc
AU  - Symons M
FAU - Vande Woude, George F
AU  - Vande Woude GF
FAU - Knudsen, Beatrice S
AU  - Knudsen BS
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.11.1 (AKT1 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/physiology
MH  - Cell Movement/*physiology
MH  - Cell Polarity
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - Enzyme Inhibitors/metabolism
MH  - Epithelial Cells/*physiology
MH  - Extracellular Matrix Proteins/metabolism
MH  - Hepatocyte Growth Factor/metabolism
MH  - Humans
MH  - Male
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Prostate/*cytology/metabolism
MH  - *Protein Serine-Threonine Kinases
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-akt
MH  - Signal Transduction/physiology
MH  - Stromal Cells/*metabolism
EDAT- 2003/08/14 05:00
MHDA- 2003/10/18 05:00
CRDT- 2003/08/14 05:00
PHST- 2003/08/14 05:00 [pubmed]
PHST- 2003/10/18 05:00 [medline]
PHST- 2003/08/14 05:00 [entrez]
AID - S0014482703002040 [pii]
AID - 10.1016/s0014-4827(03)00204-0 [doi]
PST - ppublish
SO  - Exp Cell Res. 2003 Aug 15;288(2):246-56. doi: 10.1016/s0014-4827(03)00204-0.