PMID- 12915578
OWN - NLM
STAT- MEDLINE
DCOM- 20030924
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 77
IP  - 17
DP  - 2003 Sep
TI  - Chromosomal distribution of endogenous Jaagsiekte sheep retrovirus proviral 
      sequences in the sheep genome.
PG  - 9662-8
AB  - A family of endogenous retroviruses (enJSRV) closely related to Jaagsiekte sheep 
      retrovirus (JSRV) is ubiquitous in domestic and wild sheep and goats. Southern 
      blot hybridization studies indicate that there is little active replication or 
      movement of the enJSRV proviruses in these species. Two approaches were used to 
      investigate the distribution of proviral loci in the sheep genome. Fluorescence 
      in situ hybridization (FISH) to metaphase chromosome spreads using viral DNA 
      probes was used to detect loci on chromosomes. Hybridization signals were 
      reproducibly detected on seven sheep chromosomes and eight goat chromosomes in 
      seven cell lines. In addition, a panel of 30 sheep-hamster hybrid cell lines, 
      each of which carries one or more sheep chromosomes and which collectively 
      contain the whole sheep genome, was examined for enJSRV sequences. DNA from each 
      of the lines was used as a template for PCR with JSRV gag-specific primers. A PCR 
      product was amplified from 27 of the hybrid lines, indicating that JSRV gag 
      sequences are found on at least 15 of the 28 sheep chromosomes, including those 
      identified by FISH. Thus, enJSRV proviruses are essentially randomly distributed 
      among the chromosomes of sheep and goats. FISH and/or Southern blot hybridization 
      on DNA from several of the sheep-hamster hybrid cell lines suggests that loci 
      containing multiple copies of enJSRV are present on chromosomes 6 and 9. The 
      origin and functional significance of these arrays is not known.
FAU - Carlson, Jonathan
AU  - Carlson J
AD  - Department of Microbiology, Bioagricultural Sciences and Pest Management, 
      Colorado State University, Fort Collins, Colorado 80523, USA. 
      jcarlson@colostate.edu
FAU - Lyon, Monique
AU  - Lyon M
FAU - Bishop, Jeanette
AU  - Bishop J
FAU - Vaiman, Anne
AU  - Vaiman A
FAU - Cribiu, Edmond
AU  - Cribiu E
FAU - Mornex, Jean-Francois
AU  - Mornex JF
FAU - Brown, Susan
AU  - Brown S
FAU - Knudson, Dennis
AU  - Knudson D
FAU - DeMartini, James
AU  - DeMartini J
FAU - Leroux, Caroline
AU  - Leroux C
LA  - eng
GR  - 1R01 CA 59116/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chromosomes/genetics/virology
MH  - Cricetinae
MH  - DNA, Viral/genetics/isolation & purification
MH  - Genes, gag
MH  - Genome
MH  - Goats/genetics/virology
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Jaagsiekte sheep retrovirus/*genetics/*isolation & purification
MH  - Physical Chromosome Mapping
MH  - Polymerase Chain Reaction
MH  - Proviruses/genetics/isolation & purification
MH  - Sheep/*genetics/*virology
PMC - PMC187430
EDAT- 2003/08/14 05:00
MHDA- 2003/09/25 05:00
PMCR- 2003/09/01
CRDT- 2003/08/14 05:00
PHST- 2003/08/14 05:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/08/14 05:00 [entrez]
PHST- 2003/09/01 00:00 [pmc-release]
AID - 2364 [pii]
AID - 10.1128/jvi.77.17.9662-9668.2003 [doi]
PST - ppublish
SO  - J Virol. 2003 Sep;77(17):9662-8. doi: 10.1128/jvi.77.17.9662-9668.2003.