PMID- 12915685
OWN - NLM
STAT- MEDLINE
DCOM- 20030911
LR  - 20151119
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 88
IP  - 8
DP  - 2003 Aug
TI  - In situ analysis of human menin in normal and neoplastic pancreatic tissues: 
      evidence for differential expression in exocrine and endocrine cells.
PG  - 3893-901
AB  - Multiple endocrine neoplasia type 1 (MEN1) is a hereditary syndrome linked to 
      mutations in the MEN1 gene, which encodes a 610-amino-acid nuclear protein termed 
      menin. Because of the lack of a suitable detection protocol, the in situ 
      expression pattern of menin in human tissues remains to be determined. In this 
      study, we have developed an antimenin monoclonal antibody and an indirect 
      immunofluorescence/laser-scanning microscopy protocol for analyzing menin 
      expression in frozen tissue sections. Because neuroendocrine pancreatic tumors 
      represent a key feature of MEN1, we focused this study on nontumoral pancreas and 
      a small panel of neuroendocrine pancreatic tumors. Results showed that menin was 
      readily detected in nontumoral exocrine cells. In contrast, most islet cells 
      expressing insulin, glucagon, or somatostatin showed considerably weaker levels 
      of menin expression; however, a subpopulation of pancreatic polypeptide-positive 
      cells exhibited a signal comparable with that detected in adjacent exocrine 
      cells. Sporadic endocrine tumors showed variable levels of menin expression, 
      whereas a MEN1-/- gastrinoma scored negative. This report thus provides the first 
      description of the expression pattern of menin in human pancreas in situ and lays 
      the groundwork for further studies of other tissues and tumors.
FAU - Cavallari, Ilaria
AU  - Cavallari I
AD  - Department of Oncology and Surgical Sciences, University of Padova, I-35128 
      Padova, Italy.
FAU - D'Agostino, Donna M
AU  - D'Agostino DM
FAU - Ferro, Tiziana
AU  - Ferro T
FAU - Rosato, Antonio
AU  - Rosato A
FAU - Barzon, Luisa
AU  - Barzon L
FAU - Pasquali, Claudio
AU  - Pasquali C
FAU - Fogar, Paola
AU  - Fogar P
FAU - Theodoropoulou, Marily
AU  - Theodoropoulou M
FAU - Esposito, Giovanni
AU  - Esposito G
FAU - Boscaro, Marco
AU  - Boscaro M
FAU - Pagotto, Uberto
AU  - Pagotto U
FAU - Tebaldi, Elisabetta
AU  - Tebaldi E
FAU - Fallo, Francesco
AU  - Fallo F
FAU - Chieco-Bianchi, Luigi
AU  - Chieco-Bianchi L
FAU - Ciminale, Vincenzo
AU  - Ciminale V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aged
MH  - Antibodies, Monoclonal
MH  - DNA/genetics
MH  - Diabetes Mellitus, Type 2/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gastrinoma/metabolism
MH  - Humans
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Insulinoma/metabolism
MH  - Islets of Langerhans/cytology/*metabolism
MH  - Male
MH  - Microscopy, Confocal
MH  - Neoplasm Proteins/*analysis/genetics
MH  - Pancreas/cytology/*metabolism
MH  - Pancreatic Neoplasms/*metabolism
MH  - *Proto-Oncogene Proteins
EDAT- 2003/08/14 05:00
MHDA- 2003/09/13 05:00
CRDT- 2003/08/14 05:00
PHST- 2003/08/14 05:00 [pubmed]
PHST- 2003/09/13 05:00 [medline]
PHST- 2003/08/14 05:00 [entrez]
AID - 10.1210/jc.2002-021840 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2003 Aug;88(8):3893-901. doi: 10.1210/jc.2002-021840.