PMID- 12919412
OWN - NLM
STAT- MEDLINE
DCOM- 20031110
LR  - 20191107
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 5
IP  - 9
DP  - 2003 Sep
TI  - Microbial community dynamics during start-up operation of flowerpot-using 
      fed-batch reactors for composting of household biowaste.
PG  - 765-76
AB  - Microbial community changes during start-up operation of flowerpot-using 
      fed-batch reactors for composting of household biowaste were studied by quinone 
      profiling, rRNA-targeted fluorescence in situ hybridization (FISH) and 
      cultivation methods. Total and plate counts of bacteria and quinone contents in 
      the reactors increased sharply with time during the start-up period. These 
      increase patterns had two phases; the first increase occurred during 3-4 weeks 
      from the start of waste loading and the second increase was found during the 
      subsequent 4 weeks. The microbial biomass was temporally reduced between the two 
      succession phases. Ubiquinones predominated at the beginning of operation but 
      decreased sharply with time, whereas partially saturated menaquinones became 
      predominant at the fully acclimated stage. These data indicated that the major 
      constituents of microbial populations changed from ubiquinone-containing 
      Proteobacteria to Actinobacteria during the period of operation. 
      Neighbour-joining dendrograms constructed based on the quinone profile data 
      suggested that at least one month is required to establish a stable community 
      structure with the Actinobacteria predominating. The characteristic population 
      shift in the start-up process was also demonstrated by FISH probing and 16S rDNA 
      sequence comparisons of bacterial strains isolated.
FAU - Hiraishi, Akira
AU  - Hiraishi A
AD  - Department of Ecological Engineering, Toyohashi University of Technology, 
      Toyohashi 441-8580, Japan. hiraishi@eco.tut.ac.jp
FAU - Narihiro, Takashi
AU  - Narihiro T
FAU - Yamanaka, Yosuke
AU  - Yamanaka Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Quinones)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Actinobacteria/growth & development/metabolism
MH  - Bacteria/classification/*growth & development/metabolism
MH  - Base Sequence
MH  - Biodegradation, Environmental
MH  - Bioreactors/*microbiology
MH  - Chromatography, High Pressure Liquid
MH  - Colony Count, Microbial
MH  - DNA, Bacterial/isolation & purification
MH  - DNA, Ribosomal/chemistry/isolation & purification
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Proteobacteria/growth & development/metabolism
MH  - Quinones/analysis
MH  - RNA, Ribosomal, 16S/chemistry
MH  - Refuse Disposal/*methods
MH  - Sequence Homology, Nucleic Acid
EDAT- 2003/08/16 05:00
MHDA- 2003/11/11 05:00
CRDT- 2003/08/16 05:00
PHST- 2003/08/16 05:00 [pubmed]
PHST- 2003/11/11 05:00 [medline]
PHST- 2003/08/16 05:00 [entrez]
AID - 10.1046/j.1462-2920.2003.00473.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2003 Sep;5(9):765-76. doi: 10.1046/j.1462-2920.2003.00473.x.