PMID- 12919910
OWN - NLM
STAT- MEDLINE
DCOM- 20040128
LR  - 20211216
IS  - 1000-2588 (Print)
IS  - 1000-2588 (Linking)
VI  - 23
IP  - 8
DP  - 2003 Aug
TI  - [Pathogenic mechanisms of enterotoxigenic Escherichia coli in guinea pigs].
PG  - 826-9
AB  - OBJECTIVE: To explore the pathogenic mechanisms of enterotoxigenic Escherichia 
      coli (ETEC) in guinea pigs. METHODS: Pathological examination of the intestines 
      was performed in ETEC-infected guinea pigs, and the intracellular free Ca2+ 
      concentration, cytoplasmic pH, cell membrane potential and mitochondria membrane 
      potential were determined immunohistochemically and by means of flow cytometry. 
      RESULTS: The guinea pigs were sensitive to the human-origin ETEC, which caused 
      pathological changes in the small intestines such as edema, hyperemia and 
      lymphocyte infiltration, but no bacteria invasion into the epithelial cells was 
      identified. Under transmission electron microscope, the ileal epithelial cells 
      were shown with vacuolar degeneration and evidence of mitochondrial 
      proliferation; heat-labile (LT) and heat-stable (ST) toxins pervaded in the ileal 
      tissue, resulting in significant increase of intracellular free Ca2+ 
      concentration, cytoplasmic pH value and cell membrane potential, with 
      concomitance of significant decrease in mitochondrial membrane potential. 
      CONCLUSIONS: ETEC disturbs the absorption and secretion functions of the 
      intestinal epithelial cells, and induces extensive inflammation in the small 
      intestines of guinea pigs. Besides their action on intestinal epithelial cells, 
      LT and ST also affect the myocytes in the muscularis, and their action sites and 
      mechanism might be similar. ETEC-induced diarrhea is correlated with excessive 
      water excretion from the cells.
FAU - Chen, Qing
AU  - Chen Q
AD  - Department of Epidemiology, First Military Medical University, Guangzhou 510515, 
      China. qingchen@fimmu.com
FAU - Yu, Shou-yi
AU  - Yu SY
FAU - Shen, Hong
AU  - Shen H
FAU - Wang, Ya-xian
AU  - Wang YX
FAU - Wu, Min
AU  - Wu M
LA  - chi
PT  - Journal Article
PL  - China
TA  - Di Yi Jun Yi Da Xue Xue Bao
JT  - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
JID - 9426110
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Bacterial Toxins/analysis
MH  - Calcium/metabolism
MH  - Enterotoxins/analysis
MH  - Escherichia coli/*pathogenicity
MH  - Escherichia coli Infections/metabolism/microbiology/pathology
MH  - *Escherichia coli Proteins
MH  - Female
MH  - Guinea Pigs
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/ultrastructure
MH  - Male
MH  - Microscopy, Electron
MH  - Sodium-Potassium-Exchanging ATPase/metabolism
EDAT- 2003/08/16 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/08/16 05:00
PHST- 2003/08/16 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/08/16 05:00 [entrez]
PST - ppublish
SO  - Di Yi Jun Yi Da Xue Xue Bao. 2003 Aug;23(8):826-9.