PMID- 12920861
OWN - NLM
STAT- MEDLINE
DCOM- 20031014
LR  - 20221114
IS  - 1535-3508 (Print)
IS  - 1535-3508 (Linking)
VI  - 1
IP  - 1
DP  - 2002 Jan-Mar
TI  - Radioimmunoscintigraphy of tumors autocrine for human met and hepatocyte growth 
      factor/scatter factor.
PG  - 56-62
AB  - Inappropriate expression of the c-met-protooncogene product (Met) and/or of its 
      ligand, hepatocyte growth factor/scatter factor (HGF/SF), has been correlated 
      with poor prognosis in a variety of human solid tumors. We are developing animal 
      models for nuclear imaging of Met and HGF/SF expression in tumors in vivo. We 
      radioiodinated a mixture of monoclonal antibodies (MAbs) that bind to human 
      HGF/SF and to the external ligand-binding domain of human Met, and then injected 
      the I-125-MAb mixture intravenously into mice bearing tumors either autocrine for 
      human HGF/SF and human Met or autocrine-paracrine for murine HGF/SF and murine 
      Met. Serial total body gamma camera images were obtained, and regional activity 
      was determined by quantitative region-of-interest (ROI) analysis. Tumors 
      autocrine for human HGF/SF and Met demonstrated significantly more rapid uptake 
      and more rapid clearance of the I-125-MAb mixture than tumors expressing one or 
      both murine homologues, reaching a mean tumor to total body activity ratio of > 
      0.3 by 1 day postinjection. We conclude that radioimmunodetection of tumors 
      autocrine for human HGF/SF and Met is feasible with an I-125-MAb mixture reactive 
      against the ligand-receptor pair.
FAU - Hay, Rick V
AU  - Hay RV
AD  - Van Andel Research Institute, 333 Bostwick N.E., Grand Rapids, MI 49503, USA.
FAU - Cao, Brian
AU  - Cao B
FAU - Skinner, R Scot
AU  - Skinner RS
FAU - Wang, Ling-Mei
AU  - Wang LM
FAU - Su, Yanli
AU  - Su Y
FAU - Resau, James H
AU  - Resau JH
FAU - Vande Woude, George F
AU  - Vande Woude GF
FAU - Gross, Milton D
AU  - Gross MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Imaging
JT  - Molecular imaging
JID - 101120118
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Iodine Radioisotopes)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Binding Sites
MH  - Hepatocyte Growth Factor/*analysis
MH  - Humans
MH  - Iodine Radioisotopes
MH  - Mice
MH  - Mice, Transgenic
MH  - Neoplasms/*diagnostic imaging
MH  - Proto-Oncogene Proteins c-met/*analysis
MH  - Radioimmunodetection/*methods
MH  - Tumor Cells, Cultured
EDAT- 2003/08/19 05:00
MHDA- 2003/10/15 05:00
CRDT- 2003/08/19 05:00
PHST- 2003/08/19 05:00 [pubmed]
PHST- 2003/10/15 05:00 [medline]
PHST- 2003/08/19 05:00 [entrez]
AID - 10.1162/15353500200200006 [doi]
PST - ppublish
SO  - Mol Imaging. 2002 Jan-Mar;1(1):56-62. doi: 10.1162/15353500200200006.