PMID- 12921553
OWN - NLM
STAT- MEDLINE
DCOM- 20040204
LR  - 20061115
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 38
IP  - 7
DP  - 2003 Jul
TI  - [Inhibitory effect of NS398 on the proliferation of human ovarian cancer cell 
      lines CAOV3 and OVCAR3 in vitro].
PG  - 415-8
AB  - OBJECTIVE: To observe the inhibitive effect of cyclooxygenase-2 (COX-2) selective 
      inhibitor, [N-[2-(cyclohexyloxy)-4-nitrophenyl]-methane sulfonamide] (NS398, one 
      of nonsteroidal anti-inflammatory drugs), on human ovarian cancer cell lines 
      CAOV3 and OVCAR3 during the proliferative process in vitro. METHODS: Streptovidin 
      peroxidase conjugated (SP) immunohistochemical assay was performed to examine the 
      expression of COX-2 protein in human ovarian epithelial serous cancer cell lines 
      CAOV3 and OVCAR3 respectively. The proliferative inhibition process of the two 
      cell lines by NS398 was observed with methyl thiazolyl tetrazolium (MTT) rapid 
      photocolorimetric assay. Cell morphologic changes were observed under the 
      inverted phase contrast microscope and electron microscope. DNA ladder assay and 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay were employed for the cell apoptosis. RESULTS: COX-2 protein was expressed 
      in both of the cell lines. The inhibitory rate of proliferation exerted by NS398 
      increased with the density and the time of NS398 respectively. In contrast to the 
      control, the absorbance of the experiment (NS398 200 micro mol/L for 72 h) 
      decreased obviously (P < 0.05). With regard to the cell morphology, the "vacuole" 
      presented in the cytoplasm and apoptosis body was able to be observed, and the 
      microvilli on the surface of the cell disappeared. There were characteristic 
      ladder bands after CAOV3 and OVCAR3 were treated by NS398 (100 micro mol/L) for 
      72 h. The brown-yellow granules located in the nucleus of most CAOV3 cells, which 
      indicated apoptosis. CONCLUSIONS: Above findings suggest that COX-2 may provide 
      an effective chemotherapeutic target for ovarian cancer, and selective COX-2 
      inhibitors can be used as chemotherapeutic agents for ovarian cancer.
FAU - Wang, Chun-yan
AU  - Wang CY
AD  - Department of Obstetrics and Gynecology, Second Affiliated Hospital, China 
      Medical University, Shenyang 110001, China.
FAU - Wang, Min
AU  - Wang M
FAU - Wang, Xin-yan
AU  - Wang XY
FAU - Zhang, Shu-lan
AU  - Zhang SL
FAU - Wang, Yi-xun
AU  - Wang YX
FAU - Li, Lian-kun
AU  - Li LK
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Cyclooxygenase Inhibitors)
RN  - 0 (Nitrobenzenes)
RN  - 0 (Sulfonamides)
RN  - 123653-11-2 (N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide)
SB  - IM
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Division/drug effects
MH  - Cyclooxygenase Inhibitors/*pharmacology
MH  - Female
MH  - Humans
MH  - Nitrobenzenes/*pharmacology
MH  - Ovarian Neoplasms/*drug therapy/pathology
MH  - Sulfonamides/*pharmacology
EDAT- 2003/08/19 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/08/19 05:00
PHST- 2003/08/19 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/08/19 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2003 Jul;38(7):415-8.