PMID- 12921695
OWN - NLM
STAT- MEDLINE
DCOM- 20070905
LR  - 20190916
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 15
IP  - 4
DP  - 2003
TI  - Effects of chronic prenatal ethanol exposure on NMDA receptor number and affinity 
      for [3H]MK-801 in the cerebral cortex of the young postnatal and adult 
      guinea-pig.
PG  - 207-14
AB  - The objective of this study was to test the hypothesis that chronic prenatal 
      ethanol exposure (CPEE) produces changes in the number and/or affinity of 
      N-methyl-D-aspartate (NMDA) receptors in the cerebral cortex that are 
      developmental-age-dependent. Timed, pregnant Dunkin-Hartley-strain guinea-pigs 
      received oral intubation of one of the following regimens, given daily as two 
      equally divided doses 2 h apart, from gestational day (GD) 2 to GD 67 (term, ~GD 
      68): (i) 4 g ethanol kg(-1) maternal bodyweight; (ii) isocaloric sucrose with 
      pair feeding; or (iii) water. Maternal blood ethanol concentration was measured 
      on GD 57 or 58 at 1 h after the daily dose, and was 51.1 +/- 8.5 mM (235 +/- 39 
      mg dL(-1); n = 8). At postnatal day (PD) 11 (pre-weaning) and PD 61 (adulthood), 
      body, brain and cerebral cortical weights of the offspring were measured. The 
      number of NMDA receptors and their affinity for [(3)H]MK-801 were measured in a 
      crude cerebral cortical membrane preparation using saturation isotherm analysis 
      to determine the B(max) and K(D). Chronic prenatal ethanol exposure decreased 
      offspring brain and cerebral cortical weights at PD 11 and PD 61. At PD 11, there 
      was no CPEE-induced change of [(3)H]MK-801 binding characteristics in the 
      cerebral cortex. At PD 61, both B(max) and K(D) for [(3)H]MK-801 binding to 
      cerebral cortical NMDA receptors were decreased by CPEE compared with the 
      isocaloric sucrose/pair-fed and water treatment groups. Loss of cerebral cortical 
      NMDA receptors and increased affinity of the remaining receptors for [(3)H]MK-801 
      in the adult guinea-pig, compared with no change in the number or affinity of 
      these receptors in the young postnatal offspring, demonstrated that the effects 
      of CPEE on these ionotropic glutamate receptors are developmental-age-dependent.
FAU - Puri, Rajan K
AU  - Puri RK
AD  - Department of Pharmacology and Toxicology, Faculty of Health Sciences, Queen's 
      University, Kingston, Ontario, Canada.
FAU - Reynolds, James N
AU  - Reynolds JN
FAU - Brien, James F
AU  - Brien JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Teratogens)
RN  - 3K9958V90M (Ethanol)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
SB  - IM
MH  - Animals
MH  - Cerebral Cortex/chemistry/*drug effects
MH  - Dizocilpine Maleate/*metabolism/pharmacology
MH  - Ethanol/blood/*toxicity
MH  - Excitatory Amino Acid Antagonists/*metabolism/pharmacology
MH  - Female
MH  - Guinea Pigs
MH  - Pregnancy
MH  - Receptors, N-Methyl-D-Aspartate/analysis/*drug effects/metabolism
MH  - Teratogens/*toxicity
EDAT- 2003/08/19 05:00
MHDA- 2007/09/06 09:00
CRDT- 2003/08/19 05:00
PHST- 2003/03/04 00:00 [received]
PHST- 2003/05/01 00:00 [accepted]
PHST- 2003/08/19 05:00 [pubmed]
PHST- 2007/09/06 09:00 [medline]
PHST- 2003/08/19 05:00 [entrez]
AID - RD03022 [pii]
AID - 10.1071/rd03022 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2003;15(4):207-14. doi: 10.1071/rd03022.