PMID- 12921727
OWN - NLM
STAT- MEDLINE
DCOM- 20040427
LR  - 20190901
IS  - 0021-9975 (Print)
IS  - 0021-9975 (Linking)
VI  - 129
IP  - 2-3
DP  - 2003 Aug-Oct
TI  - Expression of interleukin-10 and interleukin-12 in piglets experimentally 
      infected with porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 205-12
AB  - The expression of mRNA encoding interleukin-10 (IL-10) and IL-12 was studied, by 
      the reverse transcription-polymerase chain reaction and by in-situ hybridization 
      with a non-radioactive digoxigenin-labelled cDNA probe, in formalin-fixed, 
      paraffin wax-embedded lung tissue from piglets inoculated intranasally with a 
      Korean isolate (North American genotype) of porcine reproductive and respiratory 
      syndrome virus (PRRSV). IL-12p35-positive cells were detected in the lung at 1 
      day post-inoculation (dpi), their number increasing at 5 dpi, and rapidly 
      decreasing thereafter. In contrast, IL-10- and IL-12p40-positive cells were 
      detected in the lung at 1 dpi, their number increasing at 3 dpi, and rapidly 
      decreasing thereafter. Hybridization signals for IL-10, IL-12p35 and IL-12p40 
      were always associated with inflammation. Expression of these cytokines was 
      minimal in non-lesional lung of PRRSV-infected piglets and in normal lung from 
      control piglets. In-situ hybridization in serial sections of lung tissues 
      indicated close co-localization of PRRSV and these cytokines in interstitial 
      pneumonia. The results suggest that the expression of IL-10 and IL-12 plays a 
      role in pulmonary defence mechanisms against PRRSV infection.
FAU - Chung, H-K
AU  - Chung HK
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, San 56-1, Shillim-Dong, Kwanak-Gu 151-742, Seoul, South 
      Korea.
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Gene Expression Regulation, Viral
MH  - In Situ Hybridization
MH  - Interleukin-10/genetics/*metabolism
MH  - Interleukin-12/genetics/*metabolism
MH  - Lung/*metabolism/pathology/virology
MH  - Lung Diseases, Interstitial/metabolism/pathology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism/pathology/transmission
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - RNA, Messenger/metabolism
MH  - RNA, Viral/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
EDAT- 2003/08/19 05:00
MHDA- 2004/04/28 05:00
CRDT- 2003/08/19 05:00
PHST- 2003/08/19 05:00 [pubmed]
PHST- 2004/04/28 05:00 [medline]
PHST- 2003/08/19 05:00 [entrez]
AID - S0021997503000367 [pii]
AID - 10.1016/s0021-9975(03)00036-7 [doi]
PST - ppublish
SO  - J Comp Pathol. 2003 Aug-Oct;129(2-3):205-12. doi: 10.1016/s0021-9975(03)00036-7.