PMID- 12921944
OWN - NLM
STAT- MEDLINE
DCOM- 20031104
LR  - 20220408
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 27
IP  - 12
DP  - 2003 Dec
TI  - Comparison of interphase FISH and metaphase cytogenetics to study myelodysplastic 
      syndrome: an Eastern Cooperative Oncology Group (ECOG) study.
PG  - 1085-90
AB  - Cytogenetic analysis can be important in determining the prognosis and diagnosis 
      of a number of hematological disorders, including myelodysplastic syndromes 
      (MDS). Here, we compared metaphase chromosomal analyses on bone marrow aspirates 
      from MDS patients with interphase fluorescence in situ hybridization (FISH) using 
      probes specific for chromosomes nos. 5, 7, 8, 11, 13 and 20. Forty-three patients 
      enrolled in ECOG protocol E1996 for low risk MDS and five patients enrolled in 
      ECOG protocol E3996 for high risk MDS were studied by both metaphase chromosomal 
      analysis and interphase FISH. Excluding those with a clonal loss of the Y 
      chromosome, an abnormal clone was detected by cytogenetic analysis in 18 of 48 
      samples (37.5%). In comparison, our FISH panel detected an abnormal clone in 17 
      of 48 samples (35.4%). Twenty-nine of 30 samples with apparently normal 
      karyotypes, including those with a missing Y chromosome, were also normal by our 
      FISH panel. One patient had an occult deletion of chromosome 11 that was detected 
      by FISH. These results indicate that around 60% of patients with MDS do not have 
      abnormalities that are detectable by either chromosomal or FISH studies. In 
      addition, it appears that interphase FISH studies are nearly as sensitive as 
      cytogenetic analyses and can be a useful tool in studying bone marrow aspirates 
      where cytogenetic analysis is not possible.
FAU - Cherry, Athena M
AU  - Cherry AM
AD  - Department of Pathology, Stanford Hospital and Clinics, Stanford, CA 94305, USA. 
      athena.cherry@medventer.stanford.edu
FAU - Brockman, Stephanie R
AU  - Brockman SR
FAU - Paternoster, Sarah F
AU  - Paternoster SF
FAU - Hicks, Gary A
AU  - Hicks GA
FAU - Neuberg, Donna
AU  - Neuberg D
FAU - Higgins, Rodney R
AU  - Higgins RR
FAU - Bennett, John M
AU  - Bennett JM
FAU - Greenberg, Peter L
AU  - Greenberg PL
FAU - Miller, Kenneth
AU  - Miller K
FAU - Tallman, Martin S
AU  - Tallman MS
FAU - Rowe, Jacob
AU  - Rowe J
FAU - Dewald, Gordon W
AU  - Dewald GW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Antineoplastic Agents)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Antineoplastic Agents/therapeutic use
MH  - Bone Marrow/pathology
MH  - Chromosome Aberrations
MH  - Chromosome Banding
MH  - *Chromosomes, Human
MH  - Clinical Trials as Topic
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Karyotyping
MH  - Metaphase/*genetics
MH  - Myelodysplastic Syndromes/*genetics/pathology
EDAT- 2003/08/19 05:00
MHDA- 2003/11/05 05:00
CRDT- 2003/08/19 05:00
PHST- 2003/08/19 05:00 [pubmed]
PHST- 2003/11/05 05:00 [medline]
PHST- 2003/08/19 05:00 [entrez]
AID - S0145212603001048 [pii]
AID - 10.1016/s0145-2126(03)00104-8 [doi]
PST - ppublish
SO  - Leuk Res. 2003 Dec;27(12):1085-90. doi: 10.1016/s0145-2126(03)00104-8.