PMID- 12924435
OWN - NLM
STAT- MEDLINE
DCOM- 20030926
LR  - 20061115
IS  - 0344-0338 (Print)
IS  - 0344-0338 (Linking)
VI  - 199
IP  - 6
DP  - 2003
TI  - Lasermicrodissection--an important prerequisite for the molecular-genetic 
      analysis of bladder cancer.
PG  - 355-62
AB  - The past 10 years have shown that a crucial point in the molecular-genetic 
      analysis of malignant tumors is the exact histopathological definition and 
      separation of the investigated lesions. Laser microdissection has become an 
      important tool allowing for the study of specific histological entities and 
      defined preneoplastic lesions. Reliable detection of tumor-specific alterations 
      can be compromised by the presence of normal cells. This requires microdissection 
      of pure tumor cell populations (>80%), necessary for detecting chromosomal 
      alterations by loss of heterozygosity analysis (LOH) and fluorescence in situ 
      hybridization (FISH). The combination of microdissection and molecular methods 
      could also be useful for studying multifocal lesions of the urothelial tract. 
      This article describes in detail the use of laser microdissection, whole genome 
      amplification by Improved Primer Extension Preamplification (I-PEP)-PCR and 
      subsequent LOH, FISH, and sequencing analyses for the investigation of urothelial 
      tumors and their precursors, e.g. dysplasias and hyperplasias. The combination of 
      the described methods allows for a wide spectrum of molecular investigations in 
      lesions with a limited number of tumor cells, and might help to understand the 
      fundamental alterations involved in urothelial carcinogenesis.
FAU - Stoehr, Robert
AU  - Stoehr R
AD  - Institute of Pathology, University of Regensburg, Germany.
FAU - Wild, Peter
AU  - Wild P
FAU - Hartmann, Arndt
AU  - Hartmann A
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Chromosome Aberrations
MH  - DNA/*analysis
MH  - DNA Mutational Analysis
MH  - Dissection/*methods
MH  - Humans
MH  - Hyperplasia/genetics/pathology
MH  - In Situ Hybridization, Fluorescence
MH  - *Lasers
MH  - Loss of Heterozygosity
MH  - Microsatellite Repeats
MH  - Polymerase Chain Reaction
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 2003/08/20 05:00
MHDA- 2003/09/27 05:00
CRDT- 2003/08/20 05:00
PHST- 2003/08/20 05:00 [pubmed]
PHST- 2003/09/27 05:00 [medline]
PHST- 2003/08/20 05:00 [entrez]
AID - S0344-0338(04)70429-X [pii]
AID - 10.1078/0344-0338-00431 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2003;199(6):355-62. doi: 10.1078/0344-0338-00431.