PMID- 12927139
OWN - NLM
STAT- MEDLINE
DCOM- 20040406
LR  - 20191107
IS  - 1055-7903 (Print)
IS  - 1055-7903 (Linking)
VI  - 28
IP  - 3
DP  - 2003 Sep
TI  - Molecular genetics of Rhabdomys pumilio subspecies boundaries: mtDNA 
      phylogeography and karyotypic analysis by fluorescence in situ hybridization.
PG  - 564-75
AB  - The phylogeography of the African four-striped mouse, Rhabdomys pumilio, was 
      investigated using complete sequences of the mtDNA cytochrome b gene (1140 bp) 
      and a combination of fluorescence in situ hybridization (FISH) and conventional 
      cytogenetic banding techniques (G- and C-banding). Two cytotypes (2n=46 and 
      2n=48) were identified by cytogenetic analysis. There is no evidence of diploid 
      number variation within populations, difference in gross chromosome morphology or 
      of subtle interchromosomal rearrangements at levels detected by ZOO-FISH. 
      Analysis of the mtDNA cytochrome b resulted in two major lineages that correspond 
      roughly to the xeric and mesic biotic zones of southern Africa. One mtDNA clade 
      comprises specimens with 2n=48 and the other representatives of two cytotypes 
      (2n=48 and 2n=46). The mean sequence divergence (12%, range 8.3-15.6%) separating 
      the two mtDNA clades is comparable to among-species variation within murid genera 
      suggesting their recognition as distinct species, the prior names for which would 
      be R. dilectus and R. pumilio. Low sequence divergences and the diploid number 
      dichotomy within the mesic lineage support the recognition of two subspecies 
      corresponding to R. d. dilectus (2n=46) and R. d. chakae (2n=48). Our data do not 
      support subspecific delimitation within the nominate, R. pumilio. Molecular 
      dating places cladogenesis of the two putative species at less than five million 
      years, a period characterised by extensive climatic oscillations which are 
      thought to have resulted in habitat fragmentation throughout much of the species 
      range.
FAU - Rambau, Ramugondo V
AU  - Rambau RV
AD  - Department of Zoology, Stellenbosch University, Private Bag XI, Matieland 76021, 
      South Africa.
FAU - Stanyon, Roscoe
AU  - Stanyon R
FAU - Robinson, Terence J
AU  - Robinson TJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Phylogenet Evol
JT  - Molecular phylogenetics and evolution
JID - 9304400
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Mitochondrial)
SB  - IM
EIN - Mol Phylogenet Evol. 2004 May;31(2):805
MH  - Animals
MH  - Base Sequence
MH  - Cluster Analysis
MH  - DNA Primers
MH  - DNA, Mitochondrial/genetics
MH  - *Environment
MH  - *Evolution, Molecular
MH  - Geography
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Muridae/*genetics
MH  - *Phylogeny
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - South Africa
EDAT- 2003/08/21 05:00
MHDA- 2004/04/07 05:00
CRDT- 2003/08/21 05:00
PHST- 2003/08/21 05:00 [pubmed]
PHST- 2004/04/07 05:00 [medline]
PHST- 2003/08/21 05:00 [entrez]
AID - S1055790303000587 [pii]
AID - 10.1016/s1055-7903(03)00058-7 [doi]
PST - ppublish
SO  - Mol Phylogenet Evol. 2003 Sep;28(3):564-75. doi: 10.1016/s1055-7903(03)00058-7.