PMID- 12927358
OWN - NLM
STAT- MEDLINE
DCOM- 20031016
LR  - 20190819
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 144
IP  - 2
DP  - 2003 Sep 30
TI  - Benzo(a)pyrene induced micronucleus formation was modulated by persistent organic 
      pollutants (POPs) in metabolically competent human HepG2 cells.
PG  - 143-50
AB  - Due to their bioaccumulation and their biological properties persistent organic 
      pollutants (POPs) attract wide attention. In the present study we investigated 
      the genotoxicity, cogenotoxicity and antigenotoxicity of three selected POPs 
      (DDT, aroclor-1254 and toxaphene) in the HepG2 micronucleus assay. Exposure of 
      HepG2 cells to DDT (17.8-60 microM) and aroclor-1254 (23-184 microM) alone did 
      not increase the micronucleus-frequencies. A slight genotoxic effect could be 
      observed after exposure to toxaphene (20-40 microM). Additionally, the ability of 
      POPs to enhance/decrease the benzo(a)pyrene (BaP)-induced micronucleus formation 
      was investigated. Exposure of HepG2 cells to 50 microM BaP alone led to a more 
      than 2-fold increase of micronuclei (MN) compared with the background frequency. 
      But when the cells were pretreated with 23-181 microM aroclor-1254 or 10-20 
      microM toxaphene, BaP exposure caused significantly more MN than BaP alone. In 
      contrast, DDT (17.8-60 microM) reduced BaP-induced micronucleus induction by 
      6-38%. Mechanisms of action are discussed.
FAU - Wu, Xin-jiang
AU  - Wu XJ
AD  - Institute of Indoor and Environmental Toxicology, Justus-Liebig-University of 
      Giessen, University Hospital, Aulweg 123, D-35385 Giessen, Germany.
FAU - Lu, Wen-qing
AU  - Lu WQ
FAU - Mersch-Sundermann, Volker
AU  - Mersch-Sundermann V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Environmental Pollutants)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Insecticides)
RN  - 0 (Mutagens)
RN  - 0 (Organic Chemicals)
RN  - 11097-69-1 (Chlorodiphenyl (54% Chlorine))
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 8001-35-2 (Toxaphene)
RN  - CIW5S16655 (DDT)
SB  - IM
MH  - Benzo(a)pyrene/*toxicity
MH  - Cell Line
MH  - Chlorodiphenyl (54% Chlorine)/toxicity
MH  - DDT/toxicity
MH  - Environmental Pollutants/*toxicity
MH  - Humans
MH  - Indicators and Reagents
MH  - Insecticides/toxicity
MH  - Liver/drug effects/*metabolism
MH  - Liver Neoplasms/genetics/metabolism
MH  - *Micronucleus Tests
MH  - Mutagens/*toxicity
MH  - Organic Chemicals/*toxicity
MH  - Toxaphene/toxicity
EDAT- 2003/08/21 05:00
MHDA- 2003/10/17 05:00
CRDT- 2003/08/21 05:00
PHST- 2003/08/21 05:00 [pubmed]
PHST- 2003/10/17 05:00 [medline]
PHST- 2003/08/21 05:00 [entrez]
AID - S037842740300198X [pii]
AID - 10.1016/s0378-4274(03)00198-x [doi]
PST - ppublish
SO  - Toxicol Lett. 2003 Sep 30;144(2):143-50. doi: 10.1016/s0378-4274(03)00198-x.