PMID- 12927380
OWN - NLM
STAT- MEDLINE
DCOM- 20031030
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 190
IP  - 3
DP  - 2003 Aug 28
TI  - Allergenicity evaluation of p-chloro-m-cresol and p-chloro-m-xylenol by 
      non-radioactive murine local lymph-node assay and multiple-dose guinea pig 
      maximization test.
PG  - 259-66
AB  - p-Chloro-m-cresol (PCMC) and p-chloro-m-xylenol (PCMX) are known to cause 
      allergic contact dermatitis. For risk assessment of skin sensitizers, information 
      on dose-response profiles in the induction and elicitation phases and 
      cross-reactivity with analogous chemicals are important. In the non-radioactive 
      local lymph-node assay (LLNA) using 5-bromo-2'-deoxyuridine instead of 3H-methyl 
      thymidine, significant effect on lymph node cell proliferation was detected at 
      10% PCMC and 25% PCMX, while in the multiple-dose guinea pig maximization test 
      (GPMT) at least one animal tested in the group was sensitized at a 5 ppm 
      induction dose of either chemical. When mean skin reaction score in an animal 
      group maximally sensitized with each allergen with the GPMT was plotted against 
      log challenge concentration, linear regression lines with high correlations were 
      obtained in both cases. The calculated elicitation threshold was lower for PCMC 
      than PCMX. The area under the linear regression line between the threshold point 
      and 1% of the elicitation concentration, another index of relative elicitation 
      potency, was also greater for PCMC. Bidirectional cross-reactivity between PCMX 
      and PCMC was detected in the GPMT. PCMC was thus identified in both LLNA and GPMT 
      as a stronger sensitizer than PCMX in both the induction and elicitation phases. 
      These results suggest that the non-radioactive LLNA is a simple and useful method 
      for evaluating allergenicity in the induction phase, while the GPMT using a 
      maximally sensitized animal group is more suitable for assessing the 
      dose-response profile and cross-reactivity in the elicitation phase.
FAU - Yamano, Tetsuo
AU  - Yamano T
AD  - Osaka City Institute of Public Health, 8-34 Tojo-cho, Tennoji-ku, 543-0026 Osaka, 
      Japan. tetsuo.yamno@iphes.city.osaka.jp
FAU - Shimizu, Mitsuru
AU  - Shimizu M
FAU - Noda, Tsutomu
AU  - Noda T
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Allergens)
RN  - 0 (Cresols)
RN  - 0 (Disinfectants)
RN  - 0 (Xylenes)
RN  - 0F32U78V2Q (chloroxylenol)
RN  - 36W53O7109 (chlorocresol)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Cresols/*immunology
MH  - Dermatitis, Allergic Contact/*etiology/immunology
MH  - Disinfectants/*immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Female
MH  - Guinea Pigs
MH  - Linear Models
MH  - Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Toxicity Tests
MH  - Xylenes/*immunology
EDAT- 2003/08/21 05:00
MHDA- 2003/10/31 05:00
CRDT- 2003/08/21 05:00
PHST- 2003/08/21 05:00 [pubmed]
PHST- 2003/10/31 05:00 [medline]
PHST- 2003/08/21 05:00 [entrez]
AID - S0300483X03001616 [pii]
AID - 10.1016/s0300-483x(03)00161-6 [doi]
PST - ppublish
SO  - Toxicology. 2003 Aug 28;190(3):259-66. doi: 10.1016/s0300-483x(03)00161-6.