PMID- 12930443
OWN - NLM
STAT- MEDLINE
DCOM- 20031215
LR  - 20220311
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 92
IP  - 4
DP  - 2003 Sep
TI  - The effect of vascular endothelial growth factor and brain-derived neurotrophic 
      factor on cavernosal nerve regeneration in a nerve-crush rat model.
PG  - 470-5
AB  - OBJECTIVE: To test the hypothesis that an intracavernosal injection with 
      brain-derived neurotrophin factor (BDNF) and vascular endothelial growth factor 
      (VEGF) can facilitate nerve regeneration and recovery of erectile function after 
      cavernosal nerve injury. MATERIALS AND METHODS: The study included 25 
      Sprague-Dawley rats; four had a sham operation, seven bilateral nerve crushing 
      with no further intervention, and 14 bilateral nerve crushing with either an 
      immediate (seven) or delayed for 1 month (seven) intracavernosal injection with 
      BDNF+VEGF. Erectile function was assessed by cavernosal nerve electrostimulation 
      at 3 months, and neural regeneration by NADPH-diaphorase staining and tyrosine 
      hydroxylase (TH) staining of penile tissue and major pelvic ganglia (MPG). 
      RESULTS: After nerve crushing, the functional evaluation at 3 months showed a 
      lower mean (SD) intracavernosal pressure (ICP) with cavernosal nerve stimulation, 
      at 33.9 (15.3) cmH2O, than in the sham group, at 107.8 (18.1) cmH2O. With an 
      immediate injection with BDNF+VEGF the ICP was significantly higher than in the 
      controls, at 67.8 (38.5) cmH2O. Even delayed injection with BDNF+VEGF improved 
      the ICP, to 78.0 (21.8) cmH2O. Histological analysis of specimens stained for 
      NADPH and TH showed a significant change in the morphology of terminal branches 
      of the cavernosal and dorsal nerves, and the staining quality of the neurones in 
      the MPG. The number of positively stained nerve fibres tended to revert to normal 
      after treatment with BDNF+VEGF. CONCLUSION: An intracavernosal injection with 
      BDNF+VEGF appears to both prevent degeneration and facilitate regeneration of 
      neurones containing neuronal nitric oxide synthase in the MPG, dorsal nerve and 
      intracavernosal tissue. Therefore it might have therapeutic potential for 
      enhancing the recovery of erectile function after radical pelvic surgery.
FAU - Hsieh, P-S
AU  - Hsieh PS
AD  - Department of Urology, University of California, San Francisco, CA 94143, USA.
FAU - Bochinski, D J
AU  - Bochinski DJ
FAU - Lin, G T
AU  - Lin GT
FAU - Nunes, L
AU  - Nunes L
FAU - Lin, C S
AU  - Lin CS
FAU - Lue, T F
AU  - Lue TF
LA  - eng
PT  - Journal Article
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage
MH  - Erectile Dysfunction/*drug therapy
MH  - Male
MH  - Models, Animal
MH  - Nerve Regeneration/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Trauma, Nervous System/*drug therapy
MH  - Vascular Endothelial Growth Factor A/*administration & dosage
EDAT- 2003/08/22 05:00
MHDA- 2003/12/16 05:00
CRDT- 2003/08/22 05:00
PHST- 2003/08/22 05:00 [pubmed]
PHST- 2003/12/16 05:00 [medline]
PHST- 2003/08/22 05:00 [entrez]
AID - 4373 [pii]
AID - 10.1046/j.1464-410x.2003.04373.x [doi]
PST - ppublish
SO  - BJU Int. 2003 Sep;92(4):470-5. doi: 10.1046/j.1464-410x.2003.04373.x.