PMID- 12930808
OWN - NLM
STAT- MEDLINE
DCOM- 20030923
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 23
IP  - 20
DP  - 2003 Aug 20
TI  - Expression of functional tyrosine kinase B receptors by rhythmically active 
      respiratory neurons in the pre-Botzinger complex of neonatal mice.
PG  - 7685-9
AB  - Genetic loss of brain-derived neurotrophic factor (BDNF) severely disrupts 
      brainstem control of respiratory rhythmogenesis in newborn mice; however, the 
      sites at which BDNF acts to regulate respiratory rhythmogenesis are unknown. 
      Using immunochemical and multiplex RT-PCR analysis in mouse brainstem slices, we 
      report that the BDNF receptor, Tyrosine kinase B (TrkB), is strongly expressed in 
      the pre-Botzinger complex (PBC), the presumed site for rhythm generation, and 
      colocalizes with neurokinin 1 (NK1), a marker of neurons critical for breathing. 
      The period of the respiratory rhythm generated by PBC neurons in vitro was 
      increased by 30% after BDNF treatment (100 ng/ml) and not by nerve growth factor 
      (100 ng/ml) or BDNF (100 ng/ml) in the presence of the tyrosine kinase inhibitor 
      K252a (200 nm). Both synaptic and voltage-dependent properties of PBC neurons 
      were modified by BDNF. Synaptic currents underlying spontaneous rhythmic bursts 
      and glutamate-evoked currents were enhanced by 66 and 33%, respectively. BDNF 
      reduced the Ih current amplitude in rhythmic neurons by 46% and shifted its 
      activation curve by -17 mV. All neurons expressing TrkB mRNA (n = 8) also 
      expressed mRNAs for the Ih current [hyperpolarization-activated cyclic 
      nucleotide-sensitive cation nonselective channel (HCN1)], and three of four 
      NK1-positive neurons coexpressed TrkB and HCN mRNA. Six of 16 PBC neurons 
      expressed BDNF mRNA, supporting the possibility of autocrine and paracrine 
      actions of BDNF within the respiratory pattern generator. Our data demonstrate 
      that BDNF can modulate respiratory network activity through TrkB signaling in 
      rhythmic PBC neurons.
FAU - Thoby-Brisson, Muriel
AU  - Thoby-Brisson M
AD  - Laboratoire de Neurobiologie Genetique et Integrative, Institut Alfred Fessard, 
      Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France. 
      muriel.thoby-brisson@iaf.cnrs-gif.fr
FAU - Cauli, Bruno
AU  - Cauli B
FAU - Champagnat, Jean
AU  - Champagnat J
FAU - Fortin, Gilles
AU  - Fortin G
FAU - Katz, David M
AU  - Katz DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism/pharmacology
MH  - Cells, Cultured
MH  - Electric Conductivity
MH  - Excitatory Postsynaptic Potentials
MH  - Immunohistochemistry
MH  - Medulla Oblongata/anatomy & histology/physiology
MH  - Mice
MH  - Neurons/drug effects/*physiology
MH  - Patch-Clamp Techniques
MH  - Periodicity
MH  - RNA, Messenger/biosynthesis
MH  - Receptor, trkB/analysis/genetics/*metabolism
MH  - Respiratory Center/cytology/*metabolism/*physiology
MH  - Transcription, Genetic
PMC - PMC6740742
EDAT- 2003/08/22 05:00
MHDA- 2003/09/25 05:00
PMCR- 2004/02/20
CRDT- 2003/08/22 05:00
PHST- 2003/08/22 05:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/08/22 05:00 [entrez]
PHST- 2004/02/20 00:00 [pmc-release]
AID - 23/20/7685 [pii]
AID - 10.1523/JNEUROSCI.23-20-07685.2003 [doi]
PST - ppublish
SO  - J Neurosci. 2003 Aug 20;23(20):7685-9. doi: 10.1523/JNEUROSCI.23-20-07685.2003.