PMID- 12931223
OWN - NLM
STAT- MEDLINE
DCOM- 20040205
LR  - 20220408
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 17
IP  - 11
DP  - 2003 Nov
TI  - Is FISH a relevant prognostic tool in myelodysplastic syndromes with a normal 
      chromosome pattern on conventional cytogenetics? A study on 57 patients.
PG  - 2107-12
AB  - Conventional cytogenetics (CC) at clinical diagnosis shows a normal karyotype in 
      40-60% of de novo myelodysplastic syndromes (MDSs). Fluorescence in situ 
      hybridization (FISH) might detect occult aberrations in these patients. 
      Therefore, we have used FISH to check 57 MDS patients who were karyo-typically 
      normal on CC. At clinical diagnosis, FISH revealed a clonal abnormality in 18-28% 
      interphase cells from nine patients, five of whom also presented the same defect 
      on metaphase FISH. In five out of nine patients, the occult defect effected a 
      change in the international prognostic scoring system (IPSS). An abnormal FISH 
      pattern was significantly correlated with marrow blast cell percentage (P<10(-3)) 
      and IPSS (P<10(-3)). Patients with an occult abnormality showed an overall 
      survival and event-free survival significantly inferior in comparison to those of 
      patients with normal FISH (P<10(-3), P<10(-3)). Death and AML progression were 
      15- and eight-fold more frequent in FISH abnormal patients. In conclusion, occult 
      defects (1) are revealed in about 15% of CC normal MDS patients, (2) are 
      overlooked by CC either because of the poor quality of metaphases or their 
      submicroscopic nature, (3) are clinically relevant as they may cause a change in 
      the IPSS category and may identify a fraction of CC normal patients with an 
      unfavorable clinical outcome.
FAU - Bernasconi, P
AU  - Bernasconi P
AD  - Division of Hematology, Policlinico San Matteo IRCCS, University of Pavia, Italy.
FAU - Cavigliano, P M
AU  - Cavigliano PM
FAU - Boni, M
AU  - Boni M
FAU - Calatroni, S
AU  - Calatroni S
FAU - Klersy, C
AU  - Klersy C
FAU - Giardini, I
AU  - Giardini I
FAU - Rocca, B
AU  - Rocca B
FAU - Crosetto, N
AU  - Crosetto N
FAU - Caresana, M
AU  - Caresana M
FAU - Lazzarino, M
AU  - Lazzarino M
FAU - Bernasconi, C
AU  - Bernasconi C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - *Chromosome Mapping
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/therapy
MH  - Prognosis
MH  - Proportional Hazards Models
MH  - Reference Values
MH  - Treatment Outcome
EDAT- 2003/08/22 05:00
MHDA- 2004/02/06 05:00
CRDT- 2003/08/22 05:00
PHST- 2003/08/22 05:00 [pubmed]
PHST- 2004/02/06 05:00 [medline]
PHST- 2003/08/22 05:00 [entrez]
AID - 2403108 [pii]
AID - 10.1038/sj.leu.2403108 [doi]
PST - ppublish
SO  - Leukemia. 2003 Nov;17(11):2107-12. doi: 10.1038/sj.leu.2403108.