PMID- 12933863
OWN - NLM
STAT- MEDLINE
DCOM- 20030929
LR  - 20240413
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 71
IP  - 9
DP  - 2003 Sep
TI  - Cytotoxic necrotizing factor 1 of Escherichia coli stimulates 
      Rho/Rho-kinase-dependent myosin light-chain phosphorylation without inactivating 
      myosin light-chain phosphatase in endothelial cells.
PG  - 5188-93
AB  - Cytotoxic necrotizing factor 1 (CNF-1) is an exotoxin of Escherichia coli that 
      constitutively activates the GTPases Rho, Rac, and CDC42. Stimulation of Rho was 
      shown to enhance myosin light-chain (MLC) phosphorylation via Rho kinase-mediated 
      inhibition of MLC phosphatase in endothelial cells. Here we report that 3 h after 
      CNF stimulation of endothelial cells, RhoA was activated and MLC phosphorylation 
      was increased in a Rho/Rho-kinase-dependent manner, but no decrease in MLC 
      phosphatase activity could be detected. Despite continuous RhoA activation, MLC 
      phosphatase activity was doubled after 24 h of CNF stimulation, and this 
      coincided with decreased MLC phosphorylation and cell spreading. Rac was also 
      activated at 3 to 24 h but did not contribute to MLC phosphorylation, and its 
      amount gradually decreased in the CNF-stimulated cells. CDC42Hs was not activated 
      above control values by CNF. These results suggest that CNF can induce specific 
      decoupling (Rho kinase from MLC phosphatase) and deactivation events in Rho 
      GTPase signaling, potentially reflecting cellular protection mechanisms against 
      permanently active Rho GTPases.
FAU - Essler, Markus
AU  - Essler M
AD  - Institut fur Prophylaxe und Epidemiologie der Kreislaufkrankheiten, LMU Munchen, 
      80336 Munich, Germany.
FAU - Linder, Stefan
AU  - Linder S
FAU - Schell, Barbara
AU  - Schell B
FAU - Hufner, Katharina
AU  - Hufner K
FAU - Wiedemann, Agnes
AU  - Wiedemann A
FAU - Randhahn, Katharina
AU  - Randhahn K
FAU - Staddon, James M
AU  - Staddon JM
FAU - Aepfelbacher, Martin
AU  - Aepfelbacher M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cytotoxins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Myosin Light Chains)
RN  - 0 (Recombinant Proteins)
RN  - 106803-33-2 (cytotoxic necrotizing factor type 1)
RN  - 9013-26-7 (Actomyosin)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.53 (Myosin-Light-Chain Phosphatase)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
SB  - IM
MH  - Actomyosin/metabolism
MH  - Bacterial Toxins/genetics/*toxicity
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cytotoxins/genetics/*toxicity
MH  - DNA, Bacterial/genetics
MH  - Endothelium, Vascular/drug effects/metabolism
MH  - Escherichia coli/genetics/*pathogenicity
MH  - *Escherichia coli Proteins
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Kinetics
MH  - Myosin Light Chains/*metabolism
MH  - Myosin-Light-Chain Phosphatase
MH  - Phosphoprotein Phosphatases/metabolism
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Recombinant Proteins/genetics/toxicity
MH  - cdc42 GTP-Binding Protein/metabolism
MH  - rho GTP-Binding Proteins/metabolism
MH  - rho-Associated Kinases
PMC - PMC187342
EDAT- 2003/08/23 05:00
MHDA- 2003/09/30 05:00
PMCR- 2003/09/01
CRDT- 2003/08/23 05:00
PHST- 2003/08/23 05:00 [pubmed]
PHST- 2003/09/30 05:00 [medline]
PHST- 2003/08/23 05:00 [entrez]
PHST- 2003/09/01 00:00 [pmc-release]
AID - 0445 [pii]
AID - 10.1128/IAI.71.9.5188-5193.2003 [doi]
PST - ppublish
SO  - Infect Immun. 2003 Sep;71(9):5188-93. doi: 10.1128/IAI.71.9.5188-5193.2003.