PMID- 12935746
OWN - NLM
STAT- MEDLINE
DCOM- 20031119
LR  - 20190901
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 95
IP  - 3
DP  - 2003 Sep 1
TI  - Respiratory tract protection upon challenge of pigs vaccinated with attenuated 
      porcine reproductive and respiratory syndrome virus vaccines.
PG  - 187-97
AB  - In this study, the efficacy of two attenuated porcine reproductive and 
      respiratory syndrome virus (PRRSV) vaccines was assessed. The virological 
      protection in the lungs of vaccinated pigs upon challenge was studied. Also, 
      challenged pigs were exposed to lipopolysaccharide (LPS) to evaluate clinical 
      protection. Six-week-old pigs were immunized intramuscularly with commercial 
      vaccines based on either an attenuated American or an attenuated European virus 
      strain. Non-immunized pigs and pigs intramuscularly inoculated with the virulent 
      Lelystad strain were included as controls. Six weeks after immunization, pigs 
      were challenged either intratracheally or intranasally with the Lelystad strain, 
      and 3 and 6 days later intratracheally exposed to Escherichia coli LPS. After LPS 
      administration, pigs were monitored for clinical signs. At 4 and 7 days after 
      challenge, pigs were euthanized to determine virus quantities in broncho-alveolar 
      lavage (BAL) fluids and in lungs. Challenge virus was recovered from three out of 
      eight pigs that had been primo-inoculated with the Lelystad strain with titers 
      ranging between 0.3 and 3.1 log(10). Fifteen out of sixteen pigs vaccinated with 
      the attenuated American strain were positive for challenge virus and their mean 
      virus titers were similar to those of non-immunized challenge controls. Eleven 
      out of 16 pigs vaccinated with the attenuated European strain were positive for 
      challenge virus and their mean virus titers were 2.0-2.5 log(10) lower than those 
      of non-immunized challenge controls. Thus, the virological protection in the 
      lungs of vaccinated pigs upon challenge was incomplete, but was more pronounced 
      in the homologous situation. Clinical signs upon LPS exposure in both vaccinated 
      groups were not reproducible in two experiments.
FAU - Labarque, G
AU  - Labarque G
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Salisburylaan 133, 9820 Merelbeke, Belgium.
FAU - Van Gucht, S
AU  - Van Gucht S
FAU - Van Reeth, K
AU  - Van Reeth K
FAU - Nauwynck, H
AU  - Nauwynck H
FAU - Pensaert, M
AU  - Pensaert M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Bronchoalveolar Lavage Fluid/virology
MH  - Lipopolysaccharides/immunology
MH  - Lung Diseases/immunology/prevention & control/*veterinary/virology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*immunology/prevention & 
      control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Statistics, Nonparametric
MH  - Swine
MH  - Vaccination/*veterinary
MH  - Vaccines, Attenuated/immunology/therapeutic use
MH  - Viral Vaccines/*immunology/therapeutic use
EDAT- 2003/08/26 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/08/26 05:00
PHST- 2003/08/26 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/08/26 05:00 [entrez]
AID - S0378113503001573 [pii]
AID - 10.1016/s0378-1135(03)00157-3 [doi]
PST - ppublish
SO  - Vet Microbiol. 2003 Sep 1;95(3):187-97. doi: 10.1016/s0378-1135(03)00157-3.