PMID- 12935925
OWN - NLM
STAT- MEDLINE
DCOM- 20031001
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 145
IP  - 2
DP  - 2003 Sep
TI  - Analysis of microsatellite instability and X-inactivation in ovarian borderline 
      tumors lacking numerical abnormalities by comparative genomic hybridization.
PG  - 133-8
AB  - The genetic events underlying development of ovarian borderline tumors (tumors of 
      low malignant potential) are not well understood. In our previous studies of 
      microdissected samples from serous borderline tumors, comparative genomic 
      hybridization (CGH) and/or fluorescence in situ hybridization (FISH) analyses 
      showed that 3 of 13 tumors had detectable numerical abnormalities; the remaining 
      10 had none. In the present study, we examined microsatellite instability (MSI) 
      and clonality in this same set of tumors. Although absence of chromosomal 
      imbalances has been associated with the presence of MSI in some types of solid 
      tumors, the extent of MSI in borderline tumors, and its role in their 
      pathogenesis, is unclear. In our set of 13 tumors, no MSI was detected despite 
      analysis with microsatellite markers recommended by the National Cancer Institute 
      for assessment of MSI. Quantitative X-inactivation studies were informative at 
      the androgen receptor gene AR in 9 of the 13 tumors and revealed that each of the 
      9 tumors was clonal. In two patients, bilateral tumors showed identical patterns 
      of skewed X-inactivation. These studies confirm the clonality of borderline 
      tumors and suggest that some borderline tumors may develop through mechanisms 
      other than chromosomal imbalances or microsatellite instability.
FAU - Wolf, Nancy G
AU  - Wolf NG
AD  - Department of Genetics and Center for Human Genetics, Case Western Reserve 
      University and University Hospitals of Cleveland, Cleveland, OH 44106, USA. 
      ngw@po.cwru.edu
FAU - Farver, Carol
AU  - Farver C
FAU - Abdul-Karim, Fadi W
AU  - Abdul-Karim FW
FAU - Schwartz, Stuart
AU  - Schwartz S
LA  - eng
GR  - CA-67515/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Chromosomes, Human, X
MH  - DNA Repair/genetics
MH  - *Dosage Compensation, Genetic
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - *Microsatellite Repeats
MH  - Middle Aged
MH  - Ovarian Neoplasms/*genetics
EDAT- 2003/08/26 05:00
MHDA- 2003/10/02 05:00
CRDT- 2003/08/26 05:00
PHST- 2003/08/26 05:00 [pubmed]
PHST- 2003/10/02 05:00 [medline]
PHST- 2003/08/26 05:00 [entrez]
AID - S0165460803000931 [pii]
AID - 10.1016/s0165-4608(03)00093-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Sep;145(2):133-8. doi: 
      10.1016/s0165-4608(03)00093-1.