PMID- 12935927
OWN - NLM
STAT- MEDLINE
DCOM- 20031001
LR  - 20220311
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 145
IP  - 2
DP  - 2003 Sep
TI  - New highly sensitive fluorescence in situ hybridization method to detect PML/RARA 
      fusion in acute promyelocytic leukemia.
PG  - 144-51
AB  - We investigated a new fluorescence in situ hybridization (FISH) method to detect 
      PML/RARA fusion and/or anomalies of the RARA gene (alias RARalpha) in interphase 
      nuclei from patients with acute promyelocytic leukemia (APL). This method uses a 
      commercially available product with two different colored fluorescent probes to 
      detect both PML/RARA gene fusion products (double fusion signal or dual-color 
      fluorescence in situ hybridization [D-FISH]). A total of 82 bone marrow specimens 
      were studied, including 30 from normal bone marrow transplant donors, 33 from 
      patients with untreated APL, 14 from patients with treated APL, and 5 from APL 
      patients with known translocation variants or alternate translocations. The 
      signal patterns and percentage of abnormal nuclei were determined in a blinded 
      study on 500 interphase nuclei for each specimen. Based on 25 normal specimens, 
      the normal cutoff was >0.6% and >1.6% for t(15;17) and t(17;var), respectively. 
      The clinical sensitivity for this series of patients was 98% and the clinical 
      specificity was 100%. The results suggest that the new D-FISH probe set can 
      detect all t(15;17)(q22;q21) and all variant forms of this translocation 
      associated with PML and RARA. In addition, this FISH method can detect all 
      alternate translocations involving RARA and not PML. This FISH method can be used 
      both for the accurate diagnosis of APL and to monitor low levels of disease in 
      treated patients.
FAU - Brockman, Stephanie R
AU  - Brockman SR
AD  - Division of Laboratory Genetics, Mayo Clinic, 200 First Street SW, Rochester, MN 
      55905, USA.
FAU - Paternoster, Sarah F
AU  - Paternoster SF
FAU - Ketterling, Rhett P
AU  - Ketterling RP
FAU - Dewald, Gordon W
AU  - Dewald GW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein)
SB  - IM
MH  - Bone Marrow Cells/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Promyelocytic, Acute/*genetics
MH  - Neoplasm Proteins/*genetics
MH  - Oncogene Proteins, Fusion/*genetics
EDAT- 2003/08/26 05:00
MHDA- 2003/10/02 05:00
CRDT- 2003/08/26 05:00
PHST- 2003/08/26 05:00 [pubmed]
PHST- 2003/10/02 05:00 [medline]
PHST- 2003/08/26 05:00 [entrez]
AID - S016546080300061X [pii]
AID - 10.1016/s0165-4608(03)00061-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Sep;145(2):144-51. doi: 
      10.1016/s0165-4608(03)00061-x.