PMID- 12937226
OWN - NLM
STAT- MEDLINE
DCOM- 20040210
LR  - 20190513
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 18
IP  - 9
DP  - 2003 Sep
TI  - Monocyte activation and relationship to anti-proteinase 3 in acute vasculitis.
PG  - 1792-9
AB  - BACKGROUND: Monocytes have been suggested to play a role in antineutrophil 
      cytoplasmic antibody (ANCA)-positive vasculitis, but their state of activation in 
      vivo in patients is still unknown. METHODS: Twelve consecutive patients with 
      acute anti-proteinase 3 (PR3)-positive vasculitis were included prospectively, 
      and blood samples were drawn at diagnosis. As controls, peripheral blood was 
      obtained from a group of patients with acute infection (n = 12) and from healthy 
      controls (n = 12). Monocyte activation was estimated from the expression of 
      adhesion molecules (CD62L and CD11b), production of oxygen radicals and serum 
      concentrations of soluble inflammation markers and adhesion molecules 
      [intercellular adhesion molecule-1 (sICAM-1) and vascular cell adhesion 
      molecule-1 (sVCAM-1)]. RESULTS: Compared with monocytes from healthy subjects, 
      monocytes from patients with acute vasculitis expressed upregulated CD11b (P < 
      0.05) but had reduced production of oxygen radicals (P < 0.01). A high 
      concentration of anti-PR3 correlated with decreased expression of CD62L (r = 
      0.71, P = 0.009) and increased expression of CD11b (r = 0.63, P = 0.02). The 
      serum concentrations of soluble inflammation markers [soluble CD14, interleukin 
      (IL)-6, tumour necrosis factor receptor 1 (TNFR1), IL-10 and IL-8] as well as 
      soluble adhesion molecules (sVCAM-1 and sICAM-1) were increased. Monocytes in 
      patients with acute vasculitis displayed a reduced production of oxygen free 
      radicals (P < 0.01) but similar serum concentrations of soluble inflammation 
      markers and adhesion molecules, as compared with the control group of patients 
      with acute infection and negative PR3-ANCA. CONCLUSIONS: High anti-PR3 
      concentration in patients with acute vasculitis correlated with an activated 
      adhesion molecule phenotype (CD62L(low)/CD11b(high)) in circulating monocytes, 
      indicating a potential pathophysiological role for anti-PR3. An impaired 
      production of oxygen radicals in monocytes in patients with vasculitis compared 
      with those with acute infection may mirror the longer time interval from onset of 
      first symptoms to admission, in patients with vasculitis.
FAU - Wikman, Agneta
AU  - Wikman A
AD  - Department of Medicine, Division of Clinical Immunology and Transfusion Medicine 
      and Division of Nephrology, Karolinska Hospital and Karolinska Institutet, 
      Stockholm, Sweden. Wikman@hs.se
FAU - Fagergren, Anna
AU  - Fagergren A
FAU - Gunnar O Johansson, S
AU  - Gunnar O Johansson S
FAU - Lundahl, Joachim
AU  - Lundahl J
FAU - Jacobson, Stefan H
AU  - Jacobson SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.76 (Myeloblastin)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Antineutrophil Cytoplasmic/*immunology
MH  - Cell Adhesion Molecules/immunology
MH  - Cytokines/immunology
MH  - Female
MH  - Granulomatosis with Polyangiitis/immunology
MH  - Humans
MH  - Macrophage Activation/*immunology
MH  - Male
MH  - Middle Aged
MH  - Myeloblastin
MH  - Prospective Studies
MH  - Reactive Oxygen Species/immunology
MH  - Serine Endopeptidases/*immunology
MH  - Vasculitis/*immunology
EDAT- 2003/08/26 05:00
MHDA- 2004/02/11 05:00
CRDT- 2003/08/26 05:00
PHST- 2003/08/26 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2003/08/26 05:00 [entrez]
AID - 10.1093/ndt/gfg216 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2003 Sep;18(9):1792-9. doi: 10.1093/ndt/gfg216.