PMID- 12940957
OWN - NLM
STAT- MEDLINE
DCOM- 20040204
LR  - 20220318
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 35
IP  - 5
DP  - 2003 Sep
TI  - A new single-locus cytogenetic mapping system for maize (Zea mays L.): overcoming 
      FISH detection limits with marker-selected sorghum (S. propinquum L.) BAC clones.
PG  - 647-59
AB  - The development of a cytogenetic map for maize (Zea mays L.) is shown to be 
      feasible by means of a combination of resources from sorghum and oat that 
      overcome limitations of single-copy gene detection. A maize chromosome-addition 
      line of oat, OMAd9.2, provided clear images of optically isolated pachytene 
      chromosomes through a chromosome spread and painting technique. A direct labeled 
      oligonucleotide fluorescence in situ hybridization (FISH) probe MCCY specifically 
      stained the centromere. The arm ratio (long/short) for maize chromosome 9 in the 
      addition line was 1.7, comparable to the range of 1.6-2.1 previously reported for 
      maize chromosome 9. A sorghum (Sorghum propinquum L.) BAC library was screened by 
      hybridization with each of three maize core-bin-marker (CBM) probes: umc109 
      (CBM9.01), umc192/bz1 (CBM9.02), and csu54b (CBM9.08). A single BAC clone for 
      each marker was chosen; designated sCBM9.1, sCBM9.2, or sCBM9.8; and used as a 
      FISH probe on pachytene spreads from OMAd9.2. In each case, discrete FISH signals 
      were observed, and their cytogenetic positions were determined to be 9S.79 (at 
      position 79% of the length of chromosome 9 short arm) for sCBM9.1, 9S.65 for 
      sCBM9.2, and approximately 9L.95 for sCBM9.8. These map positions were co-linear 
      with linkage-map positions for these and other loci common to the linkage and 
      cytogenetic maps. This work represents a major breakthrough for cytogenetic 
      mapping of the maize genome, and also provides a general strategy that can be 
      applied to cytogenetic mapping of other plant species with relatively large and 
      complex genomes.
FAU - Koumbaris, George L
AU  - Koumbaris GL
AD  - Department of Biological Science, The Florida State University, Tallahassee, FL 
      32306-4370, USA.
FAU - Bass, Hank W
AU  - Bass HW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Avena/genetics
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Plant/genetics
MH  - Genetic Markers/genetics
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Poaceae/*genetics
MH  - Sorghum/genetics
MH  - Zea mays/genetics
EDAT- 2003/08/28 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/08/28 05:00
PHST- 2003/08/28 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/08/28 05:00 [entrez]
AID - 1829 [pii]
AID - 10.1046/j.1365-313x.2003.01829.x [doi]
PST - ppublish
SO  - Plant J. 2003 Sep;35(5):647-59. doi: 10.1046/j.1365-313x.2003.01829.x.