PMID- 12941141
OWN - NLM
STAT- MEDLINE
DCOM- 20031024
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 110
IP  - 1
DP  - 2003 Sep
TI  - Unimpaired dendritic cell functions in MVP/LRP knockout mice.
PG  - 58-65
AB  - Dendritic cells (DCs) act as mobile sentinels of the immune system. By 
      stimulating T lymphocytes, DCs are pivotal for the initiation of both T- and 
      B-cell-mediated immune responses. Recently, ribonucleoprotein particles (vaults) 
      were found to be involved in the development and/or function of human DCs. To 
      further investigate the role of vaults in DCs, we examined the effects of 
      disruption of the major vault protein (MVP/LRP) on the development and 
      antigen-presenting capacity of DCs, using our MVP/LRP knockout mouse model. 
      Mononuclear bone marrow cells were isolated from wild-type and knockout mice and 
      stimulated to differentiate to DCs. Like human DCs, the wild-type murine DC 
      cultures strongly expressed MVP/LRP. Nevertheless, the MVP/LRP-deficient DCs 
      developed normally and showed similar expression levels of several DC surface 
      markers. No differences were observed in in vitro studies on the antigen uptake 
      and presenting capacities of the wild-type and MVP/LRP knockout DCs. Moreover, 
      immunization of the MVP/LRP-deficient mice with several T-cell antigens led to 
      responses similar to those observed in the wild-type mice, indicating that the in 
      vivo DC migration and antigen-presentation capacities are intact. Moreover, no 
      differences were observed in the induction of the T cell-dependent humoral 
      responses and orally induced peripheral T-cell tolerance. In conclusion, vaults 
      are not required for primary DC functions. Their abundance in DCs may, however, 
      still reflect basic roles in myeloid cell proliferation and DC development.
FAU - Mossink, Marieke H
AU  - Mossink MH
AD  - Department of Hematology, Erasmus Medical Center, Rotterdam, the Netherlands.
FAU - de Groot, Jan
AU  - de Groot J
FAU - van Zon, Arend
AU  - van Zon A
FAU - Franzel-Luiten, Erna
AU  - Franzel-Luiten E
FAU - Schoester, Martijn
AU  - Schoester M
FAU - Scheffer, George L
AU  - Scheffer GL
FAU - Sonneveld, Pieter
AU  - Sonneveld P
FAU - Scheper, Rik J
AU  - Scheper RJ
FAU - Wiemer, Erik A C
AU  - Wiemer EA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vault Ribonucleoprotein Particles)
RN  - 0 (major vault protein)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Hypersensitivity, Delayed/immunology
MH  - Immune Tolerance
MH  - Immunoglobulin G/biosynthesis
MH  - Immunophenotyping
MH  - Lymphocyte Culture Test, Mixed
MH  - Macrophages, Peritoneal/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - T-Lymphocytes/immunology
MH  - Vault Ribonucleoprotein Particles/genetics/*immunology/metabolism
PMC - PMC1783015
EDAT- 2003/08/28 05:00
MHDA- 2003/10/25 05:00
PMCR- 2004/09/01
CRDT- 2003/08/28 05:00
PHST- 2003/08/28 05:00 [pubmed]
PHST- 2003/10/25 05:00 [medline]
PHST- 2003/08/28 05:00 [entrez]
PHST- 2004/09/01 00:00 [pmc-release]
AID - 1708 [pii]
AID - 10.1046/j.1365-2567.2003.01708.x [doi]
PST - ppublish
SO  - Immunology. 2003 Sep;110(1):58-65. doi: 10.1046/j.1365-2567.2003.01708.x.