PMID- 12941963
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 278
IP  - 45
DP  - 2003 Nov 7
TI  - A rapid increase in the total number of cell surface functional GABAA receptors 
      induced by brain-derived neurotrophic factor in rat visual cortex.
PG  - 44097-102
AB  - The number of postsynaptic gamma-aminobutyric acid type A (GABAA) receptors is a 
      fundamental determinant of the variability of inhibitory synaptic responses in 
      the central nervous system. In rat visual cortex, [3H]SR-95531 binding assays 
      revealed that brain-derived neurotrophic factor (BDNF), one of the neurotrophins, 
      induced a rapid increase in the total number of cell surface GABAA receptors, 
      through the activation of Trk B receptor tyrosine kinases. We also demonstrated 
      that BDNF rapidly induced a sustained potentiation of GABAA receptor-mediated 
      currents, using nystatin-perforated patch clamp recordings, in visual cortical 
      layer 5 pyramidal neurons freshly isolated from P14 rats. The potentiation was 
      caused by the activation of Trk B receptor tyrosine kinase and phospholipase 
      C-gamma. In addition, intracellular Ca2+ was important for the potentiation of 
      GABAA responses induced by BDNF. The selective increase in mean miniature 
      inhibitory postsynaptic (mIPSC) current amplitude without effects on mIPSC time 
      courses supports the idea that BDNF rapidly induces an increase in the total 
      number of cell surface functional GABAA receptors in visual cortical pyramidal 
      neurons. These results suggest that BDNF could alter the number of cell surface 
      GABAA receptors in a region-specific manner.
FAU - Mizoguchi, Yoshito
AU  - Mizoguchi Y
AD  - Cellular and Systems Physiology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka 812-8582, Japan.
FAU - Kanematsu, Takashi
AU  - Kanematsu T
FAU - Hirata, Masato
AU  - Hirata M
FAU - Nabekura, Junichi
AU  - Nabekura J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030826
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Pyridazines)
RN  - 0 (Receptors, GABA-A)
RN  - 10028-17-8 (Tritium)
RN  - 1400-61-9 (Nystatin)
RN  - 99460MG420 (gabazine)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Calcium/physiology
MH  - Cell Membrane/chemistry/physiology
MH  - Electric Conductivity
MH  - Enzyme Activation/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Nystatin
MH  - Patch-Clamp Techniques
MH  - Phospholipase C gamma
MH  - Pyridazines/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/metabolism
MH  - Receptors, GABA-A/analysis/*metabolism/physiology
MH  - Tritium
MH  - Type C Phospholipases/antagonists & inhibitors/metabolism
MH  - Visual Cortex/*drug effects/*metabolism/ultrastructure
EDAT- 2003/08/28 05:00
MHDA- 2004/01/06 05:00
CRDT- 2003/08/28 05:00
PHST- 2003/08/28 05:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/08/28 05:00 [entrez]
AID - S0021-9258(20)82418-1 [pii]
AID - 10.1074/jbc.M305872200 [doi]
PST - ppublish
SO  - J Biol Chem. 2003 Nov 7;278(45):44097-102. doi: 10.1074/jbc.M305872200. Epub 2003 
      Aug 26.