PMID- 12942543
OWN - NLM
STAT- MEDLINE
DCOM- 20031008
LR  - 20220318
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 197
IP  - 1
DP  - 2003 Oct
TI  - Phenotypic change regulates monocyte chemoattractant protein-1 (MCP-1) gene 
      expression in human retinal pigment epithelial cells.
PG  - 77-85
AB  - We investigated the expression profile of monocyte chemoattractant protein-1 
      (MCP-1) in human retinal pigment epithelial (RPE) cells under different culture 
      conditions and evaluated the molecular mechanism responsible for MCP-1 gene 
      expression in RPE cells. After cellular confluence, total RNA was extracted and 
      used for RT-PCR. Medium conditioned by RPE was used for ELISA and Western 
      blotting. The result showed that RPE cells cultured on plastic expressed MCP-1 
      constitutively in the absence of any stimuli. On the other hand, growing human 
      RPE on laminin-coated flasks instead of plastic reduced the production of MCP-1. 
      In the RPE cells cultured on plastic, IkappaB was degraded and A20 protein 
      increased concomitantly. MCP-1 upregulation in RPE cells on plastic was 
      attenuated by the addition of MG-132, a proteasome inhibitor. Also, the addition 
      of pyrolidine dithiocarbonate (PDTC) and hypoxic conditions (0.5% O2) decreased 
      MCP-1 production in these cells. These findings suggested that the expression 
      profile of MCP-1 is regulated by phenotypic alterations of the RPE cells. And the 
      increased MCP-1 expression in RPE cells cultured on plastic is caused via 
      spontaneous activation of NFkappaB induced by susceptibility to oxidative stress.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Uetama, Tomoko
AU  - Uetama T
AD  - Department of Ophthalmology and Visual Science, Graduate School, Tokyo Medical 
      and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan.
FAU - Ohno-Matsui, Kyoko
AU  - Ohno-Matsui K
FAU - Nakahama, Ken-Ichi
AU  - Nakahama K
FAU - Morita, Ikuo
AU  - Morita I
FAU - Mochizuki, Manabu
AU  - Mochizuki M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Laminin)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Blotting, Western
MH  - Cell Culture Techniques/methods
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/*genetics
MH  - Culture Media, Conditioned/chemistry
MH  - Cytokines/analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Gene Expression Regulation
MH  - Humans
MH  - I-kappa B Proteins/metabolism
MH  - Laminin
MH  - NF-kappa B/metabolism
MH  - Oxidative Stress
MH  - Phenotype
MH  - Pigment Epithelium of Eye/cytology/*physiology
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/08/28 05:00
MHDA- 2003/10/09 05:00
CRDT- 2003/08/28 05:00
PHST- 2003/08/28 05:00 [pubmed]
PHST- 2003/10/09 05:00 [medline]
PHST- 2003/08/28 05:00 [entrez]
AID - 10.1002/jcp.10342 [doi]
PST - ppublish
SO  - J Cell Physiol. 2003 Oct;197(1):77-85. doi: 10.1002/jcp.10342.