PMID- 12944167
OWN - NLM
STAT- MEDLINE
DCOM- 20040419
LR  - 20181130
IS  - 0265-6736 (Print)
IS  - 0265-6736 (Linking)
VI  - 19
IP  - 5
DP  - 2003 Sep-Oct
TI  - Thermal regulation of dendritic cell activation and migration from skin explants.
PG  - 520-33
AB  - Dendritic cells (DCs) in the skin rapidly take up antigen and migrate out of the 
      skin to draining lymph nodes for antigen presentation. As a result, these cells 
      play an important role in generating specific immune responses against infectious 
      agents that enter the skin and against antigens delivered as vaccines. Previous 
      efforts revealed that fever-like elevations in body temperature enhance 
      antigen-dependent immune responses initiated at the site of the skin and 
      stimulate the migration of epidermal DCs to draining lymph nodes. Collectively, 
      these data have led to the hypothesis that the activation of epidermal DCs is 
      sensitive to physiological thermal stimuli. In this study, ear skin explants 
      derived from BALB/c mice were either maintained at 37 degrees C or incubated at 
      40 degrees C for the first 6.5 h before being placed at 37 degrees C. This 
      heating protocol altered the density and morphology of the epidermal DCs in a 
      manner suggestive of an increased kinetics of activation-associated DC migration. 
      Flow cytometric analysis of the emigrated cells also indicated that mild heating 
      enhanced the migration kinetics of DCs and increased the DC expression of MHC 
      class II and the activation marker CD86. Importantly, these migrated cells 
      displayed higher stimulatory capacity in a mixed lymphocyte reaction compared to 
      those of controls. Overall, these results suggest that mild thermal stimuli can 
      enhance DC activation and function and that strategic applications of heat could 
      enhance the potency of vaccines consisting of relatively weak antigens, such as 
      cancer vaccines.
FAU - Ostberg, J R
AU  - Ostberg JR
AD  - Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA. 
      julie.ostberg@roswellpark.org
FAU - Kabingu, E
AU  - Kabingu E
FAU - Repasky, E A
AU  - Repasky EA
LA  - eng
GR  - CA 16056/CA/NCI NIH HHS/United States
GR  - CA71599/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Int J Hyperthermia
JT  - International journal of hyperthermia : the official journal of European Society 
      for Hyperthermic Oncology, North American Hyperthermia Group
JID - 8508395
RN  - 0 (Cytokines)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cell Movement/*immunology
MH  - Cytokines/genetics
MH  - Dendritic Cells/*cytology/*immunology
MH  - *Epidermal Cells
MH  - Epidermis/immunology
MH  - Female
MH  - Gene Expression
MH  - HSP70 Heat-Shock Proteins/genetics
MH  - Hot Temperature
MH  - *Hyperthermia, Induced
MH  - Kinetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Organ Culture Techniques
MH  - RNA, Messenger/analysis
MH  - Skin Temperature/immunology
MH  - Vaccination
EDAT- 2003/08/29 05:00
MHDA- 2004/04/20 05:00
CRDT- 2003/08/29 05:00
PHST- 2003/08/29 05:00 [pubmed]
PHST- 2004/04/20 05:00 [medline]
PHST- 2003/08/29 05:00 [entrez]
AID - GHXH26KXPB1VEYBK [pii]
AID - 10.1080/02656730310001607986 [doi]
PST - ppublish
SO  - Int J Hyperthermia. 2003 Sep-Oct;19(5):520-33. doi: 10.1080/02656730310001607986.