PMID- 12946860
OWN - NLM
STAT- MEDLINE
DCOM- 20040430
LR  - 20190827
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 89
IP  - 1
DP  - 2003 Oct 9
TI  - Analysis of dendritic cell trafficking using EGFP-transgenic mice.
PG  - 17-24
AB  - Dendritic cells (DCs) are professional antigen presenting cells, well equipped to 
      initiate an immune response. For effective induction of an immune response, DC 
      should migrate from the periphery to the lymph node to present the antigen to T 
      lymphocytes. Currently, tumor-antigen loaded DCs are used in clinical vaccination 
      trials in cancer patients. To investigate the migratory capacity of DC in vivo, a 
      variety of fluorescent and radioactive labels have been used. Here we introduce a 
      novel tool to study DC migration in vivo: DCs generated from enhanced green 
      fluorescent protein (EGFP)-transgenic mice. DC from EGFP-transgenic mice display 
      typical DC behavior and can be matured without affecting their autofluorescence 
      in vitro. In addition, the continuously produced cytoplasmic EGFP in living cells 
      functions as a viability marker, since EGFP released from dying cells does not 
      stain DC from C57Bl/6 mice upon coculture. In vivo migration studies using 
      EGFP-DC and indium-111-labeled DC were performed to determine the efficiency of 
      i.d. versus s.c. administered DC to reach the draining lymph node. The analysis 
      demonstrates that i.d. injection increases the amount of 
      EGFP-DC/indium-111-labeled DC in the lymph node compared to s.c. injection. 
      Subsequent quantitative, phenotypical and ultrastuctural analysis demonstrate 
      that DC generated from EGFP-transgenic mice are well suited to study the 
      migratory behavior, distribution and phenotype of DC in vivo.
FAU - Eggert, Andreas A O
AU  - Eggert AA
AD  - Tumor Immunology Laboratory, University Medical Center Nijmegen St. Radboud, P.O. 
      Box 9101, 6500 HB Nijmegen, The Netherlands.
FAU - van der Voort, Robbert
AU  - van der Voort R
FAU - Torensma, Ruurd
AU  - Torensma R
FAU - Moulin, Veronique
AU  - Moulin V
FAU - Boerman, Otto C
AU  - Boerman OC
FAU - Oyen, Wim J G
AU  - Oyen WJ
FAU - Punt, C J A
AU  - Punt CJ
FAU - Diepstra, Heleen
AU  - Diepstra H
FAU - de Boer, Annemiek J
AU  - de Boer AJ
FAU - Figdor, Carl G
AU  - Figdor CG
FAU - Adema, Gosse J
AU  - Adema GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology/*physiology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Green Fluorescent Proteins
MH  - Injections, Subcutaneous
MH  - Interleukin-4/pharmacology
MH  - Kidney/immunology
MH  - Luminescent Proteins/analysis
MH  - Lymph Nodes/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Phenotype
MH  - Recombinant Proteins
MH  - Spleen/immunology
EDAT- 2003/08/30 05:00
MHDA- 2004/05/01 05:00
CRDT- 2003/08/30 05:00
PHST- 2003/08/30 05:00 [pubmed]
PHST- 2004/05/01 05:00 [medline]
PHST- 2003/08/30 05:00 [entrez]
AID - S0165247803001056 [pii]
AID - 10.1016/s0165-2478(03)00105-6 [doi]
PST - ppublish
SO  - Immunol Lett. 2003 Oct 9;89(1):17-24. doi: 10.1016/s0165-2478(03)00105-6.