PMID- 12947002
OWN - NLM
STAT- MEDLINE
DCOM- 20040226
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 103
IP  - 2
DP  - 2004 Jan 15
TI  - Identification of novel CTL epitopes of CMV-pp65 presented by a variety of HLA 
      alleles.
PG  - 630-8
AB  - Cytomegalovirus (CMV)-specific T-cell immunity plays an important role in 
      protection from CMV disease in immunocompromised patients. Identification of 
      cytotoxic T-lymphocyte (CTL) epitopes is essential for monitoring T-cell immunity 
      and also for immunotherapy. In this and previous studies, CMV-pp65-specific CTL 
      lines were successfully generated from all of 11 CMV-seropositive healthy donors, 
      using pp65-transduced CD40-activated B (CD40-B) cells as antigen-presenting 
      cells. By use of enzyme-linked immunospot (ELISPOT) assays, individual CTL 
      epitopes could be mapped with truncated forms of the pp65 gene. For human 
      leukocyte antigen (HLA) alleles with a known binding motif, CTL epitopes within 
      the defined regions were predicted by computer algorithm. For HLA alleles without 
      a known binding motif (HLA-Cw*0801, -Cw*1202, and -Cw*1502), the epitopes were 
      alternatively identified by step-by-step truncations of the pp65 gene. Through 
      this study, a total of 14 novel CTL epitopes of CMV-pp65 were identified. 
      Interestingly, 3 peptides were found to be presented by 2 different HLA class I 
      alleles or subtypes. Moreover, use of CD40-B cells pulsed with a mixture of 
      synthetic peptides led to generation of pp65-specific CTL lines from some of 
      seronegative donors. The study thus demonstrated an efficient strategy for 
      identifying CTL epitopes presented by a variety of HLA alleles.
FAU - Kondo, Eisei
AU  - Kondo E
AD  - Division of Immunology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, 
      Chikusa-ku, Nagoya 464-8681, Japan.
FAU - Akatsuka, Yoshiki
AU  - Akatsuka Y
FAU - Kuzushima, Kiyotaka
AU  - Kuzushima K
FAU - Tsujimura, Kunio
AU  - Tsujimura K
FAU - Asakura, Shoji
AU  - Asakura S
FAU - Tajima, Kohei
AU  - Tajima K
FAU - Kagami, Yoshitoyo
AU  - Kagami Y
FAU - Kodera, Yoshihisa
AU  - Kodera Y
FAU - Tanimoto, Mitsune
AU  - Tanimoto M
FAU - Morishima, Yasuo
AU  - Morishima Y
FAU - Takahashi, Toshitada
AU  - Takahashi T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030828
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphoproteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (cytomegalovirus matrix protein 65kDa)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Viral/genetics/*immunology
MH  - B-Lymphocytes/immunology
MH  - Base Sequence
MH  - Cytomegalovirus/genetics/*immunology
MH  - Cytomegalovirus Infections/blood/*immunology
MH  - DNA Primers
MH  - Epitopes/chemistry/*immunology
MH  - Genes, Reporter
MH  - HLA-A Antigens/immunology
MH  - HLA-B Antigens/immunology
MH  - HLA-C Antigens/immunology
MH  - Humans
MH  - Mutagenesis
MH  - Mutagenesis, Site-Directed
MH  - Peptide Fragments/chemistry/immunology
MH  - Phosphoproteins/genetics/*immunology
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - Reference Values
MH  - Sequence Deletion
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Viral Matrix Proteins/genetics/*immunology
EDAT- 2003/08/30 05:00
MHDA- 2004/02/27 05:00
CRDT- 2003/08/30 05:00
PHST- 2003/08/30 05:00 [pubmed]
PHST- 2004/02/27 05:00 [medline]
PHST- 2003/08/30 05:00 [entrez]
AID - S0006-4971(20)54650-8 [pii]
AID - 10.1182/blood-2003-03-0824 [doi]
PST - ppublish
SO  - Blood. 2004 Jan 15;103(2):630-8. doi: 10.1182/blood-2003-03-0824. Epub 2003 Aug 
      28.