PMID- 12947703
OWN - NLM
STAT- MEDLINE
DCOM- 20040715
LR  - 20131121
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 34
IP  - 2
DP  - 2003 Apr
TI  - [Effects of brain-derived neurotrophic factor on H2O2-induced apoptosis of human 
      arterial smooth muscle cell].
PG  - 251-3, 259
AB  - OBJECTIVE: To investigate the protective effects of brain-derived neurotrophic 
      factor (BDNF) on hydrogen peroxide (H2O2) induced apoptosis of human arterial 
      smooth muscle cells (SMC). METHODS: The hBDNF cDNA was transfected into the SMC 
      mediated by Lipofect- AMINE, and ectopic expression in SMC was driven by the 
      regulatory elements of I type collagen gene(COLIA1). The cell proliferation was 
      measured with a colorimetric assay based on the cleavage of the tetrazolium salt 
      MTT. Microscopic analysis of cell apoptosis was performed with fluorescent stain 
      of acridine orange (AO). Apoptosis rate of SMC was tested by flow cytometry 
      (FCM). DNA pattern was examined by agarose gel electrophoresis. RESULTS: Exposure 
      of growing SMC to 100-800 mumol/L H2O2 for 24 h remarkably suppressed the cell 
      proliferation. After treatment of the cells with 200 mumol/L H2O2 for 24 h, 
      morphological and biochemical changes of classic apoptosis such as condensation 
      of cytoplasm, fragmentation of the nuclear chromatin, DNA "ladder" pattern after 
      electrophoresis were observed. Yet, the above-mentioned apoptosis changes were 
      not observed and the apoptosis rate was reduced obviously in the BDNF-transfected 
      SMC treated with the same concentration of H2O2. CONCLUSION: H2O2 can induce 
      apoptosis of SMC, but BDNF inhibits H2O2-induced SMC apoptosis markedly and has 
      strong anti-apoptotic activity.
FAU - Chen, Jianye
AU  - Chen J
AD  - Institute of Biochemistry and Molecular Biology, West China School of Preclinical 
      and Forensic Medicine, Sichuan University, Chengdu 610041, China.
FAU - Liu, Ji
AU  - Liu J
FAU - Wang, Ruohan
AU  - Wang R
FAU - Liu, Zhimin
AU  - Liu Z
FAU - Li, Ji
AU  - Li J
FAU - Chen, Junjie
AU  - Chen J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Hydrogen Peroxide
MH  - Muscle, Smooth, Vascular/*cytology
MH  - Transfection
EDAT- 2003/09/02 05:00
MHDA- 2004/07/16 05:00
CRDT- 2003/09/02 05:00
PHST- 2003/09/02 05:00 [pubmed]
PHST- 2004/07/16 05:00 [medline]
PHST- 2003/09/02 05:00 [entrez]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):251-3, 259.