PMID- 12949238
OWN - NLM
STAT- MEDLINE
DCOM- 20031027
LR  - 20190605
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 74
IP  - 3
DP  - 2003 Sep
TI  - Expression of IL-2 receptor beta and gamma chains by human gingival fibroblasts 
      and up-regulation of adhesion to neutrophils in response to IL-2.
PG  - 352-9
AB  - To investigate the role of human gingival fibroblasts (HGF), the major 
      constituents of gingival tissue in periodontal inflammatory disease, the 
      expression of interleukin-2 receptor (IL-2R) alpha, beta, and gamma chains was 
      examined. Immunohistochemistry showed a pronounced accumulation of CD8(+) T cells 
      in the inflamed lamina propria of gingival tissue from patients with adult 
      periodontitis. HGF express IL-2Rbeta and IL-2Rgamma at mRNA and protein levels, 
      but the expression of IL-2Ralpha could not be detected, as assessed by reverse 
      transcriptase-polymerase chain reaction and flow cytometry. IL-2Rbeta, and -gamma 
      expressed on HGF were functionally active, as addition of neutralizing 
      anti-IL-2Rbeta and -gamma antibodies caused inhibition of the IL-2-induced 
      production of monocyte chemoattractant protein-1 (MCP-1), and addition of IL-2 
      induced phosphorylation of Janus tyrosine kinase 3, which is critical in 
      signaling through IL-2Rgamma in HGF. The IL-2-induced MCP-1 production was 
      significantly inhibited by pretreatment with neutralizing antibody to IL-15. 
      Addition of IL-2 also induced a marked up-regulation of the expression of 
      intercellular adhesion molecule-1 (ICAM-1) on the surface of HGF, which in turn, 
      significantly augmented the adhesion of human neutrophils, which were inhibited 
      by an anti-ICAM-1 antibody. These results suggest that HGF express functional 
      IL-2Rbetagamma, respond to IL-2 from infiltrated T cells, and actively 
      participate in the inflammatory process in the periodontal region and that IL-15 
      produced by HGF sustains IL-2-mediated signaling in HGF.
FAU - Ozawa, Akiko
AU  - Ozawa A
AD  - Department of Microbiology and Immunology, Tohoku University Graduate School of 
      Dentistry, Sendai, Japan.
FAU - Tada, Hiroyuki
AU  - Tada H
FAU - Tamai, Riyoko
AU  - Tamai R
FAU - Uehara, Akiko
AU  - Uehara A
FAU - Watanabe, Kouichi
AU  - Watanabe K
FAU - Yamaguchi, Takahiro
AU  - Yamaguchi T
FAU - Shimauchi, Hidetoshi
AU  - Shimauchi H
FAU - Takada, Haruhiko
AU  - Takada H
FAU - Sugawara, Shunji
AU  - Sugawara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CD4 Antigens)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-15)
RN  - 0 (Interleukin-2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-2)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (JAK3 protein, human)
RN  - EC 2.7.10.2 (Janus Kinase 3)
SB  - IM
MH  - Adult
MH  - CD4 Antigens/pharmacology
MH  - Cell Adhesion/*drug effects
MH  - Cells, Cultured
MH  - DNA Primers/chemistry
MH  - Fibroblasts/*metabolism
MH  - Flow Cytometry
MH  - Gingiva/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-15/metabolism
MH  - Interleukin-2/*pharmacology
MH  - Janus Kinase 3
MH  - Lipopolysaccharides/pharmacology
MH  - Neutrophil Activation
MH  - Neutrophils/*metabolism
MH  - Periodontitis/metabolism
MH  - Phosphorylation
MH  - Protein Isoforms
MH  - Protein-Tyrosine Kinases/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Interleukin-2/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/immunology/metabolism
MH  - Up-Regulation
EDAT- 2003/09/02 05:00
MHDA- 2003/10/28 05:00
CRDT- 2003/09/02 05:00
PHST- 2003/09/02 05:00 [pubmed]
PHST- 2003/10/28 05:00 [medline]
PHST- 2003/09/02 05:00 [entrez]
AID - 10.1189/jlb.0103044 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2003 Sep;74(3):352-9. doi: 10.1189/jlb.0103044.