PMID- 12949243
OWN - NLM
STAT- MEDLINE
DCOM- 20031027
LR  - 20190605
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 74
IP  - 3
DP  - 2003 Sep
TI  - Lactobacilli and streptococci induce inflammatory chemokine production in human 
      macrophages that stimulates Th1 cell chemotaxis.
PG  - 395-402
AB  - Macrophages have a central role in innate-immune responses to bacteria. In the 
      present work, we show that infection of human macrophages with Gram-positive 
      pathogenic Streptococcus pyogenes or nonpathogenic Lactobacillus rhamnosus GG 
      enhances mRNA expression of inflammatory chemokine ligands CCL2/monocyte 
      chemoattractant protein-1 (MCP-1), CCL3/macrophage-inflammatory protein-1alpha 
      (MIP-1alpha), CCL5/regulated on activation, normal T expressed and secreted, 
      CCL7/MCP-3, CCL19/MIP-3beta, and CCL20/MIP-3alpha and CXC chemokine ligands 
      CXCL8/interleukin (IL)-8, CXCL9/monokine induced by interferon-gamma (IFN-gamma), 
      and CXCL10/IFN-inducible protein 10. Bacteria-induced CCL2, CCL7, CXCL9, and 
      CXCL10 mRNA expression was partially dependent on ongoing protein synthesis. The 
      expression of these chemokines and of CCL19 was dependent on bacteria-induced 
      IFN-alpha/beta production. CCL19 and CCL20 mRNA expression was up-regulated by 
      IL-1beta or tumor necrosis factor alpha (TNF-alpha), and in addition, IFN-alpha 
      together with TNF-alpha further enhanced CCL19 gene expression. Synergy between 
      IFN-alpha and TNF-alpha was also seen for CXCL9 and CXCL10 mRNA expression. 
      Bacteria-stimulated macrophage supernatants induced the migration of T helper 
      cell type 1 (Th1) cells, suggesting that in human macrophages, these bacteria can 
      stimulate efficient inflammatory chemokine gene expression including those that 
      recruit Th1 cells to the site of inflammation. Furthermore, L. rhamnosus-induced 
      Th1 chemokine production could in part explain the proposed antiallergenic 
      properties of this bacterium.
FAU - Veckman, Ville
AU  - Veckman V
AD  - Department of Microbiology, National Public Health Institute, Helsinki, Finland. 
      ville.veckman@ktl.fi
FAU - Miettinen, Minja
AU  - Miettinen M
FAU - Matikainen, Sampsa
AU  - Matikainen S
FAU - Lande, Roberto
AU  - Lande R
FAU - Giacomini, Elena
AU  - Giacomini E
FAU - Coccia, Eliana M
AU  - Coccia EM
FAU - Julkunen, Ilkka
AU  - Julkunen I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokines)
RN  - 0 (RNA, Messenger)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokines/*biosynthesis/genetics
MH  - *Chemotaxis, Leukocyte
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interferons/pharmacology
MH  - Lactobacillus/*physiology
MH  - Macrophages/metabolism/*microbiology
MH  - RNA, Messenger/analysis
MH  - Streptococcus pyogenes/*physiology
MH  - Th1 Cells/*immunology
EDAT- 2003/09/02 05:00
MHDA- 2003/10/28 05:00
CRDT- 2003/09/02 05:00
PHST- 2003/09/02 05:00 [pubmed]
PHST- 2003/10/28 05:00 [medline]
PHST- 2003/09/02 05:00 [entrez]
AID - 10.1189/jlb.0402212 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2003 Sep;74(3):395-402. doi: 10.1189/jlb.0402212.