PMID- 12949897
OWN - NLM
STAT- MEDLINE
DCOM- 20031002
LR  - 20061115
IS  - 0361-8609 (Print)
IS  - 0361-8609 (Linking)
VI  - 74
IP  - 1
DP  - 2003 Sep
TI  - Fluorescence in situ hybridization: method of choice for a definitive diagnosis 
      of mantle cell lymphoma.
PG  - 78-84
AB  - Fluorescence in situ hybridization (FISH) using IGH/CCND1 probes was used to 
      analyze 35 specimens including 27 paraffin sections, 3 bone marrow aspirates, and 
      5 peripheral blood smears. The 27 paraffin sections included 7 bone marrows, 10 
      lymph nodes, 3 spleens, 3 tonsils, 3 gastrointestinal biopsies, and 1 skin 
      biopsy. Among these cases, 23 specimens were from 20 patients with mantle cell 
      lymphoma (MCL) and 12 specimens were from 12 patients with non-MCL 
      lymphomas/lymphoid hyperplasia. Specimens from all MCL patients showed positive 
      results with FISH. In one patient, the archived paraffin sections were negative 
      with FISH, but a fresh peripheral blood specimen showed a positive result. 
      Negative results were obtained in all specimens from non-MCL cases. Flow 
      cytometric analysis revealed that all cases of MCL showed CD19/CD5 staining, but 
      the percentages of cells positive for CD23 and FMC-7 were variable, thus they 
      cannot be depended upon for a definitive diagnosis of MCL. Immunohistochemical 
      stains demonstrated positive staining for CD5 and CD20 and negative staining for 
      CD23 in MCL cases but cyclin D1 was positive in only 10 of 13 MCL cases studied. 
      Therefore, it appears that immunophenotyping alone is not sufficient to establish 
      a definitive diagnosis of MCL. FISH should be routinely used when the diagnosis 
      needs confirmation. FISH can be performed in a routine clinical laboratory, and 
      it is applicable to archived material for retrospective studies. Other molecular 
      cytogenetic techniques in comparison with FISH are discussed.
FAU - Sun, Tsieh
AU  - Sun T
AD  - Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center, 
      Denver, Colorado 80220, USA. Tsieh.Jack.Sun@med.va.gov
FAU - Nordberg, Mary Lowery
AU  - Nordberg ML
FAU - Cotelingam, James D
AU  - Cotelingam JD
FAU - Veillon, Diana M
AU  - Veillon DM
FAU - Ryder, John
AU  - Ryder J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Am J Hematol
JT  - American journal of hematology
JID - 7610369
SB  - IM
MH  - Diagnosis, Differential
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphoma, Mantle-Cell/*diagnosis
EDAT- 2003/09/02 05:00
MHDA- 2003/10/03 05:00
CRDT- 2003/09/02 05:00
PHST- 2003/09/02 05:00 [pubmed]
PHST- 2003/10/03 05:00 [medline]
PHST- 2003/09/02 05:00 [entrez]
AID - 10.1002/ajh.10356 [doi]
PST - ppublish
SO  - Am J Hematol. 2003 Sep;74(1):78-84. doi: 10.1002/ajh.10356.