PMID- 12950019
OWN - NLM
STAT- MEDLINE
DCOM- 20031024
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 201
IP  - 1
DP  - 2003 Sep
TI  - Association of C-MYC amplification with progression from the in situ to the 
      invasive stage in C-MYC-amplified breast carcinomas.
PG  - 75-82
AB  - Human carcinoma in situ of the breast already demonstrates genomic changes found 
      in invasive lesions. However, no specific genetic alterations have previously 
      been identified that are associated with progression from the in situ to the 
      invasive stage. By comparative genomic hybridization (CGH) and fluorescence in 
      situ hybridization (FISH) analysis of an invasive breast carcinoma with a large 
      associated in situ component, high-level amplification of C-MYC was found in the 
      invasive component only. To determine the frequency of this correlation in a 
      panel of 188 invasive breast carcinomas, 18 additional cases with C-MYC 
      amplification were identified. Nine of these cases had a detectable adjacent in 
      situ component. FISH analysis demonstrated increased (>5) C-MYC signals per 
      nucleus in seven invasive components and increased (>4) C-MYC/centromere 8 signal 
      ratios in five of these. None of the associated in situ components demonstrated 
      these increases. The minimal amplified region was defined at 8q24.13-8qter. C-MYC 
      amplification was correlated with overexpression of C-MYC and two of its target 
      genes, TERT and FBL. Thus, C-MYC amplification is the first identified genetic 
      alteration that is associated with progression from the in situ to the invasive 
      stage of breast carcinoma.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Robanus-Maandag, Els C
AU  - Robanus-Maandag EC
AD  - Division of Experimental Therapy, The Netherlands Cancer Institute, Amsterdam, 
      The Netherlands.
FAU - Bosch, Cathy A J
AU  - Bosch CA
FAU - Kristel, Petra M
AU  - Kristel PM
FAU - Hart, Augustinus A M
AU  - Hart AA
FAU - Faneyte, Ian F
AU  - Faneyte IF
FAU - Nederlof, Petra M
AU  - Nederlof PM
FAU - Peterse, Johannes L
AU  - Peterse JL
FAU - van de Vijver, Marc J
AU  - van de Vijver MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*genetics/pathology
MH  - Carcinoma in Situ/*genetics/pathology
MH  - Carcinoma, Intraductal, Noninfiltrating/*genetics/pathology
MH  - Chromosomes, Human, Pair 8/genetics
MH  - DNA, Neoplasm/genetics
MH  - Disease Progression
MH  - Female
MH  - Gene Amplification
MH  - *Genes, myc
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction/methods
EDAT- 2003/09/02 05:00
MHDA- 2003/10/25 05:00
CRDT- 2003/09/02 05:00
PHST- 2003/09/02 05:00 [pubmed]
PHST- 2003/10/25 05:00 [medline]
PHST- 2003/09/02 05:00 [entrez]
AID - 10.1002/path.1385 [doi]
PST - ppublish
SO  - J Pathol. 2003 Sep;201(1):75-82. doi: 10.1002/path.1385.