PMID- 12950105
OWN - NLM
STAT- MEDLINE
DCOM- 20040416
LR  - 20191210
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 66
IP  - 2
DP  - 2003 Oct
TI  - A new FISH assay to simultaneously detect structural and numerical chromosomal 
      abnormalities in mouse sperm.
PG  - 172-80
AB  - De novo aberrations in chromosome structure represent important categories of 
      paternally transmitted genetic damage. Unlike numerical abnormalities, the 
      majority of de novo structural aberrations among human offspring are of paternal 
      origin. We report the development of a three-color fluorescence in situ 
      hybridization (FISH) assay (CT8) to detect mouse sperm carrying structural and 
      numerical chromosomal abnormalities. The CT8 assay uses DNA probes for the 
      centromeric and telomeric regions of chromosome 2, and a probe for the 
      subcentromeric region of chromosome 8. The CT8 assay was used to measure the 
      frequencies of sperm carrying certain structural aberrations involving chromosome 
      2 (del2ter, dup2ter, del2cen, dup2cen), disomy 2, disomy 8, and sperm diploidy. 
      Analysis of approximately 80,000 sperm from eight B6C3F1 mice revealed an average 
      baseline frequency of 2.5 per 10,000 sperm carrying partial duplications and 
      deletions of chromosome 2. Extrapolated to the entire haploid genome, 
      approximately 0.4% of mouse sperm are estimated to carry structural chromosomal 
      aberrations, which is more than fivefold lower than the spontaneous frequencies 
      of sperm with chromosome structural aberrations in man. We validated the CT8 
      assay by comparing the frequencies of abnormal segregants in sperm of T(2;14) 
      translocation carriers detected by this assay against those detected by 
      chromosome painting cytogenetic analysis of meiosis II spermatocytes. The CT8 
      sperm FISH assay is a promising method for detecting structural chromosome 
      aberrations in mouse sperm with widespread applications in genetics, physiology, 
      and genetic toxicology.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Hill, Francesca S
AU  - Hill FS
AD  - Biology and Biotechnology Research Program, Lawrence Livermore National 
      Laboratory, Livermore, California, USA.
FAU - Marchetti, Francesco
AU  - Marchetti F
FAU - Liechty, Melissa
AU  - Liechty M
FAU - Bishop, Jack
AU  - Bishop J
FAU - Hozier, John
AU  - Hozier J
FAU - Wyrobek, Andrew J
AU  - Wyrobek AJ
LA  - eng
GR  - Y01-ES-8016/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Validation Study
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Centromere/ultrastructure
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Mammalian/*ultrastructure
MH  - DNA Probes
MH  - Epididymis
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Mice
MH  - Spermatozoa/*ultrastructure
MH  - Translocation, Genetic
EDAT- 2003/09/02 05:00
MHDA- 2004/04/17 05:00
CRDT- 2003/09/02 05:00
PHST- 2003/09/02 05:00 [pubmed]
PHST- 2004/04/17 05:00 [medline]
PHST- 2003/09/02 05:00 [entrez]
AID - 10.1002/mrd.10299 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2003 Oct;66(2):172-80. doi: 10.1002/mrd.10299.