PMID- 12950454
OWN - NLM
STAT- MEDLINE
DCOM- 20031017
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 86
IP  - 6
DP  - 2003 Sep
TI  - Cloning and characterization of the 3'-untranslated region of the human 
      excitatory amino acid transporter 2 transcript.
PG  - 1458-67
AB  - The 3'-untranslated region (UTR) of the human excitatory amino acid transporter 2 
      (EAAT2) transcript was cloned and characterized. The full-length EAAT2 cDNA of 11 
      692 bp was found to contain 283 bp of 5' UTR, a 1725-bp open reading frame and an 
      unusually long 3'-UTR of 9684 bp. The 3'-UTR of EAAT2 cDNA was well conserved 
      among mammals, and human, macaque, rat and mouse cDNA had nearly identical 3' 
      ends. The human EAAT2 transcripts were detected in brain, spinal cord, liver, 
      adrenal gland, placenta and pancreas by northern hybridization, and many ESTs 
      homologous to the human EAAT2 cDNA were found in numerous tissues. To investigate 
      the role of human EAAT2 3'-UTR in gene expression, we constructed luciferase 
      expression vectors containing 3'-UTR fragments spanning the entire length of the 
      region. The individual fragments varied in their effects on reporter gene 
      expression in human astrocytes by a factor of eight to ten suggesting a complex 
      role of the 3'-UTR in post-transcriptional regulation of EAAT2 gene expression.
FAU - Kim, Seon-Young
AU  - Kim SY
AD  - Molecular Virology Division, St Luke's-Roosevelt Hospital Center, New York, New 
      York, USA.
FAU - Chao, Wei
AU  - Chao W
FAU - Choi, So-Young
AU  - Choi SY
FAU - Volsky, David J
AU  - Volsky DJ
LA  - eng
GR  - P01 NS031492/NS/NINDS NIH HHS/United States
GR  - NS31492/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (3' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 3' Untranslated Regions/chemistry/*genetics
MH  - Animals
MH  - Astrocytes/metabolism
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA, Complementary/genetics
MH  - Excitatory Amino Acid Transporter 2/*genetics
MH  - Expressed Sequence Tags
MH  - Gene Expression Regulation/physiology
MH  - Genes, Reporter
MH  - Genetic Vectors
MH  - Humans
MH  - Macaca
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - RNA, Messenger/chemistry/*genetics
MH  - Rats
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Analysis, RNA
MH  - Sequence Homology, Nucleic Acid
EDAT- 2003/09/03 05:00
MHDA- 2003/10/18 05:00
CRDT- 2003/09/03 05:00
PHST- 2003/09/03 05:00 [pubmed]
PHST- 2003/10/18 05:00 [medline]
PHST- 2003/09/03 05:00 [entrez]
AID - 1958 [pii]
AID - 10.1046/j.1471-4159.2003.01958.x [doi]
PST - ppublish
SO  - J Neurochem. 2003 Sep;86(6):1458-67. doi: 10.1046/j.1471-4159.2003.01958.x.