PMID- 12950598
OWN - NLM
STAT- MEDLINE
DCOM- 20031028
LR  - 20191107
IS  - 1083-4389 (Print)
IS  - 1083-4389 (Linking)
VI  - 8
IP  - 4
DP  - 2003 Aug
TI  - Surface localized Heat Shock Protein 20 (HslV) of Helicobacter pylori.
PG  - 257-67
AB  - BACKGROUND: Heat Shock Protein (HSP) has been regarded as a pathogenic factor in 
      Helicobacter pylori infection. Heat Shock Protein 20 (HSP20) of H. pylori is 
      identified as Hs1V based on open reading frame predication of genome sequences. 
      It is a homologue of HslV of E. coli, a peptidase involved in protein 
      degradation. METHODS: The HSP20 gene was cloned and inserted into pET16b fused 
      with His-tag. Recombinant HSP20 protein (rHSP20) was expressed and purified by 
      nickel column. Rabbit anti-rHSP20 was purified by Protein A affinity 
      chromatography and used as a probe to localize HSP20 in H. pylori by immuno-gold 
      labeling and Western blotting. rHSP20 was also used as antigen to test for 
      antibody against HSP20 in patients with H. pylori infection by enzyme-linked 
      immunosorbant assay. RESULTS: Immuno-gold labeled transmission electron 
      microscopy shows that HSP20 is located on the cell surface of H. pylori. Western 
      blotting of 2-D gel shows that HSP20 has a pI of approximately 5.5 and a 
      molecular weight of approximately 18 kDa. The ELISA result shows that there is no 
      significant difference in antibody titre against rHSP20 in all sera tested. 
      CONCLUSION: The presence of IgG to rHSP20 may imply an earlier exposure of the 
      patients and normal subjects to H. pylori. However, the mechanism has not been 
      established. HSP20 has been shown to localize on the surface of H. pylori. 
      Surface localization of H. pylori HSP20 may provide the path to a better 
      understanding of the role and function of HSP20 in bacteria-host interaction.
FAU - Du, Rui Juan
AU  - Du RJ
AD  - Department of Microbiology, National University of Singapore, 5 Science Drive 2, 
      Singapore 117597.
FAU - Ho, Bow
AU  - Ho B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Helicobacter
JT  - Helicobacter
JID - 9605411
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Bacterial/blood
MH  - Bacterial Proteins/*analysis/chemistry/immunology/isolation & purification
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cell Membrane/chemistry
MH  - Cloning, Molecular
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Genes, Bacterial
MH  - Heat-Shock Proteins/*analysis/chemistry/immunology/isolation & purification
MH  - Helicobacter pylori/*chemistry/*ultrastructure
MH  - Humans
MH  - Microscopy, Immunoelectron
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/analysis/chemistry/immunology/isolation & purification
EDAT- 2003/09/03 05:00
MHDA- 2003/10/29 05:00
CRDT- 2003/09/03 05:00
PHST- 2003/09/03 05:00 [pubmed]
PHST- 2003/10/29 05:00 [medline]
PHST- 2003/09/03 05:00 [entrez]
AID - 153 [pii]
AID - 10.1046/j.1523-5378.2003.00153.x [doi]
PST - ppublish
SO  - Helicobacter. 2003 Aug;8(4):257-67. doi: 10.1046/j.1523-5378.2003.00153.x.